School of Chemical Engineering, Yeungnam University, Gyeongsan-si Gyeongsangbuk-do 712-749, Korea.
Curr Microbiol. 2011 Apr;62(4):1321-30. doi: 10.1007/s00284-010-9862-4. Epub 2011 Jan 9.
Two lineages of enterohemorrhagic Escherichia coli O157:H7 (EDL933, Stx1(+) and Stx2(+)) and 86-24 (Stx2(+)) were investigated to determine the genetic basis of biofilm formation on abiotic surfaces. Strain EDL933 formed a robust biofilm while strain 86-24 formed almost no biofilm on either polystyrene plates or polyethylene tubes. Whole-transcriptome profiles of EDL933 versus 86-24 revealed that in the strong biofilm-forming strain, genes involved in curli biosynthesis and cellulose production were significantly induced, whereas genes involved in indole signaling were most repressed. Additionally, 49 phage genes were highly induced and repressed between the two strains. Curli assays using Congo red plates and scanning electron microscopy corroborated the microarray data as the EDL933 strain produced a large amount of curli, while strain 86-24 formed much less curli. Moreover, EDL933 produced 19-fold more cellulose than 86-24, and indole production in EDL933 was two times lower than that of the strain 86-24. Therefore, it appears E. coli O157:H7 EDL933 produces more biofilm because of its increased curli and cellulose production and reduced indole production.
研究了两株肠出血性大肠杆菌 O157:H7（EDL933，Stx1(+) 和 Stx2(+)）和 86-24（Stx2(+)），以确定其在非生物表面形成生物膜的遗传基础。EDL933 菌株形成了一个强大的生物膜，而 86-24 菌株在聚苯乙烯板或聚乙烯管上几乎没有形成生物膜。EDL933 与 86-24 的全转录组图谱显示，在强生物膜形成菌株中，卷曲生物合成和纤维素产生相关的基因显著诱导，而吲哚信号相关的基因则受到最大程度的抑制。此外，两株菌株之间有 49 个噬菌体基因被高度诱导和抑制。使用刚果红平板和扫描电子显微镜进行的卷曲菌试验证实了微阵列数据，因为 EDL933 菌株产生了大量的卷曲菌，而 86-24 菌株形成的卷曲菌则少得多。此外，EDL933 产生的纤维素比 86-24 多 19 倍，而 EDL933 中的吲哚产量比 86-24 低两倍。因此，肠出血性大肠杆菌 O157:H7 EDL933 似乎由于其卷曲菌和纤维素产量的增加以及吲哚产量的降低而产生更多的生物膜。
