National Food Research Institute, Tsukuba-Shi, Ibaraki, Japan.
FEMS Microbiol Ecol. 2011 May;76(2):220-35. doi: 10.1111/j.1574-6941.2011.01044.x. Epub 2011 Feb 1.
We prepared DNA from the production waters of oil deposits and wellheads of the high- and hypertemperature Japanese oil wells #AR39 (depth, 1230 m; temperature, 74 °C; pressure, 2.92 MPa) and #SR123 (depth, 1687 m; temperature, 98 °C; pressure, 11.3 MPa) to detect indigenous bacterial and archaeal microorganisms. We used PCR to amplify the 16S rRNA genes of microbial communities and characterized them based on their sequences. A few species of microorganisms with high GC contents were detected in samples from oil deposits, whereas the microbial constituents and their GC contents were diverse in wellhead samples. A comparison of the composition of the microbial communities found that the predominant indigenous populations in the #SR123 oil deposit were Thermotoga hypogea-, Thermotoga petrophila- and Thermodesulfobacterium commune-like bacteria with a 61-63% GC content in their 16S rRNA gene sequences, and Archaeoglobus fulgidus-like archaea with a 65% GC content, whereas the major population in #AR39 comprised Thermacetogenium phaeum- and Fervidobacterium pennavorans-like bacteria and Methanothermobacter thermautotrophicus-like archaea with a 60%, 60% and 61% GC content, respectively.
我们从日本高温高压油井 #AR39（深度 1230 米；温度 74°C；压力 2.92 MPa）和 #SR123（深度 1687 米；温度 98°C；压力 11.3 MPa）的产油层和井口采集了 DNA，以检测本土细菌和古菌微生物。我们使用 PCR 扩增微生物群落的 16S rRNA 基因，并根据其序列对其进行特征分析。在油藏样本中检测到了一些 GC 含量较高的微生物种类，而井口样本中的微生物组成及其 GC 含量则多种多样。对微生物群落组成的比较表明，#SR123 油藏中主要的本土种群是 Thermotoga hypogea-、Thermotoga petrophila- 和 Thermodesulfobacterium commune 样细菌，其 16S rRNA 基因序列的 GC 含量为 61-63%，而古菌则是 Archaeoglobus fulgidus 样，GC 含量为 65%，而 #AR39 中的主要种群则是 Thermacetogenium phaeum-、Fervidobacterium pennavorans 样细菌和 Methanothermobacter thermautotrophicus 样古菌，其 GC 含量分别为 60%、60%和 61%。
