Department of Gynecology and Gynecologic Oncology, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
BMC Cancer. 2011 Jan 11;11:12. doi: 10.1186/1471-2407-11-12.
Carbonic anhydrase IX (CAIX) is a membranous expressed metalloenzyme involved in pH homeostasis and cell adhesion. The protein is overexpressed in a variety of tumors and potentially associated with negative outcome. This study was designed to investigate the prognostic role of CAIX in serum and tumor tissue of patients with primary cervical cancer.
Tumor samples of 221 consecutive patients with primary cervical cancer who underwent surgery between 1993 and 2008 were analyzed for CAIX expression by immunohistochemistry. Additionally, preoperative serum CAIX concentrations were determined by ELISA in a subset of patients. Correlation with intratumoral CAIX expression as well as clinicopathological factors and outcome was analyzed.
CAIX expression was observed in 81.9% of the tumor specimens; 62.0% showed a moderate or strong staining intensity. Moderate/strong expression was associated with squamous histology (p=0.024), advanced tumor stage (p=0.001), greater invasion depth (p=0.025), undifferentiated tumor grade (p<0.001) and high preoperative SCC-Ag values (p=0.042). Furthermore patients with moderate/strong intratumoral CAIX expression had a higher number of metastatic lymph nodes compared to those with none/weak intratumoral expression levels (p=0.047) and there was a non-significant association between high intratumoral CAIX expression and shorter survival (p=0.118). Preoperative serum concentrations of CAIX ranged between 23 and 499 pg/mL and did not correlate with intratumoral expression or other clinicopathological variables.
CAIX is associated with advanced tumor stages and lymph node metastases in cervical cancer, potentially representing a new target in this disease. In contrast to other epithelial cancers we could not observe a correlation between serum CAIX and its intratumoral expression.
碳酸酐酶 IX（CAIX）是一种膜结合的金属酶，参与 pH 平衡和细胞黏附。该蛋白在多种肿瘤中过度表达，可能与不良预后相关。本研究旨在探讨 CAIX 在原发性宫颈癌患者血清和肿瘤组织中的预后作用。
对 1993 年至 2008 年间接受手术治疗的 221 例原发性宫颈癌患者的肿瘤标本进行免疫组织化学分析，检测 CAIX 表达。在部分患者中，通过 ELISA 检测术前血清 CAIX 浓度。分析 CAIX 表达与肿瘤内 CAIX 表达、临床病理因素及预后的相关性。
81.9%的肿瘤标本中观察到 CAIX 表达；62.0%显示中等或强染色强度。中等/强表达与鳞状组织学（p=0.024）、晚期肿瘤分期（p=0.001）、更深的浸润深度（p=0.025）、未分化的肿瘤分级（p<0.001）和高术前 SCC-Ag 值（p=0.042）相关。此外，中等/强肿瘤内 CAIX 表达的患者与无/弱肿瘤内表达水平的患者相比，转移淋巴结的数量更多（p=0.047），而高肿瘤内 CAIX 表达与生存时间较短之间无显著相关性（p=0.118）。术前血清 CAIX 浓度范围为 23-499pg/ml，与肿瘤内表达或其他临床病理变量无关。
CAIX 与宫颈癌的晚期肿瘤分期和淋巴结转移相关，可能成为该疾病的新靶点。与其他上皮癌不同，我们未观察到血清 CAIX 与其肿瘤内表达之间的相关性。
