与纯化的小牛轮状病毒相关的核糖核酸聚合酶活性

Ribonucleic acid polymerase activity associated with purified calf rotavirus.

作者信息

Cohen J

出版信息

J Gen Virol. 1977 Sep;36(3):395-402. doi: 10.1099/0022-1317-36-3-395.

Abstract

The presence of an RNA-dependent RNA polymerase is demonstrated in purified rotavirus particles. Optimum polymerase activity was found between 45 to 50 degrees C, at pH 8, and in the presence of 10 mM-magnesium ions. The polymerase product was highly sensitive to pancreatic RNase (97%) in low or high salt concentration. The enzyme was activated by EDTA treatment of intact particles or heat shock. The similarities between reovirus, blue-tongue virus and rotavirus polymerases are discussed.

摘要

在纯化的轮状病毒颗粒中证实了存在一种依赖RNA的RNA聚合酶。发现最佳聚合酶活性在45至50摄氏度、pH值为8且存在10 mM镁离子的条件下。聚合酶产物在低盐或高盐浓度下对胰核糖核酸酶高度敏感（97%）。该酶通过对完整颗粒进行EDTA处理或热休克而被激活。文中讨论了呼肠孤病毒、蓝舌病毒和轮状病毒聚合酶之间的相似性。