Bacha Abir Ben
Biochemistry Department, Science College, King Saud University, P.O Box 22452, Riyadh, 11495, Saudi Arabia.
Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, Sfax, 3038, Tunisia.
Appl Biochem Biotechnol. 2014 Oct;174(4):1520-1534. doi: 10.1007/s12010-014-1069-x. Epub 2014 Aug 15.
Group IIA secreted phospholipase A2 (group IIA sPLA2) is known to display potent Gram-positive bactericidal activity in vitro and in vivo. We have analyzed the bactericidal activity of the full set of native stingray and dromedary groups V, IIA, and IB sPLA2s on several Gram-positive and Gram-negative strains. The rank order potency among both marine and mammal sPLA2s against Gram-positive bacteria is group IIA > V > IB, whereas Gram-negative bacteria exhibited a much higher resistance. There is a synergic action of the sPLA2 with lysozyme when added to the bacteria culture prior to sPLA2.The bactericidal efficiency of groups V and IIA sPLA2s was shown to be dependent upon the presence of calcium ions and to a less extent Mg(2+) ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Importantly, we showed that stingray and dromedary groups V, IIA, and IB sPLA2s present no cytotoxicity after their incubation with MDA-MB-231cells. stingray groups V and IIA sPLA2s, like mammal ones, may be considered as future therapeutic agents against bacterial infections.
IIA 组分泌型磷脂酶 A2（IIA 组 sPLA2）已知在体外和体内均表现出强大的革兰氏阳性杀菌活性。我们分析了天然黄貂鱼和单峰骆驼的 V 组、IIA 组和 IB 组 sPLA2 全套对几种革兰氏阳性和革兰氏阴性菌株的杀菌活性。海洋和哺乳动物 sPLA2 对革兰氏阳性菌的效力排序为 IIA 组＞V 组＞IB 组，而革兰氏阴性菌表现出更高的抗性。当在 sPLA2 之前添加到细菌培养物中时，sPLA2 与溶菌酶存在协同作用。V 组和 IIA 组 sPLA2 的杀菌效率显示取决于钙离子的存在，在较小程度上还取决于 Mg(2+)离子，然后可以将其与膜磷脂的水解活性建立关联。重要的是，我们表明黄貂鱼和单峰骆驼的 V 组、IIA 组和 IB 组 sPLA2 与 MDA-MB-231 细胞孵育后无细胞毒性。黄貂鱼的 V 组和 IIA 组 sPLA2，与哺乳动物的一样，可被视为未来抗细菌感染的治疗剂。
