嗜热自养甲烷杆菌中超氧化物歧化酶基因的特性分析

Characterization of a superoxide dismutase gene from the archaebacterium Methanobacterium thermoautotrophicum.

作者信息

Takao M, Oikawa A, Yasui A

机构信息

Department of Bioscience, School of Hygienic Science, Kitasato University, Sagamihara, Japan.

出版信息

Arch Biochem Biophys. 1990 Nov 15;283(1):210-6. doi: 10.1016/0003-9861(90)90633-a.

DOI:10.1016/0003-9861(90)90633-a

PMID:2122808

Abstract

A gene encoding superoxide dismutase (SOD) was cloned from the archaebacterium Methanobacterium thermoautotrophicum, the first example from an anaerobic bacterium. The deduced amino acid sequence showed overall similarity to sequences of known Mn- and Fe-SODs from aerobic organisms. Judging from a detailed sequence comparison, the cloned SOD gene is classified as Mn-SOD. By comparison of Mn-SOD sequences among various species it was suggested that archaebacterial superoxide dismutase is a direct descendant of a primordial enzyme. Between a putative promoter and the start codon there is an inverted repeat sequence which is also found in the counterpart of Halobacterium halobium.

摘要

从嗜热自养甲烷杆菌这种古细菌中克隆出了一个编码超氧化物歧化酶（SOD）的基因，这是来自厌氧菌的首个此类实例。推导得到的氨基酸序列与来自需氧生物的已知锰超氧化物歧化酶和铁超氧化物歧化酶序列总体相似。从详细的序列比较判断，克隆得到的SOD基因被归类为锰超氧化物歧化酶。通过比较不同物种间的锰超氧化物歧化酶序列，有人提出古细菌超氧化物歧化酶是一种原始酶的直接后代。在假定的启动子和起始密码子之间存在一个反向重复序列，在嗜盐嗜盐菌的对应区域也能找到该序列。