Chae H Z, Robison K, Poole L B, Church G, Storz G, Rhee S G
Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7017-21. doi: 10.1073/pnas.91.15.7017.
A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of > 25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members.
利用针对牛硫醇特异性抗氧化酶（TSA）的抗体，从大鼠脑cDNA文库中分离出了一段与TSA对应的cDNA。该cDNA克隆编码一个能够编码198个氨基酸残基多肽的开放阅读框。大鼠和酵母的TSA蛋白与鼠伤寒沙门氏菌烷基过氧化氢还原酶的21 kDa组分（AhpC）具有显著的序列同源性，并且我们发现AhpC具有TSA活性。AhpC和TSA定义了一个存在于所有生物界生物中的、超过25种不同蛋白质的家族。家族成员之间的相似性延伸至整个序列，同一性在23%至98%之间。AhpC/TSA家族的大多数成员含有两个保守的半胱氨酸。在编码其他氧化还原酶活性的基因附近发现了至少八个编码AhpC/TSA样多肽的基因，并且一些同源物的表达与致病性相关。我们认为AhpC/TSA家族代表了一类广泛分布的抗氧化酶。我们还报告称，由烷基过氧化氢还原酶的57 kDa组分（AhpF）和硫氧还蛋白还原酶定义的第二类蛋白质家族已扩展至包括另外六个成员。
