Owhashi M, Horii Y, Ikeda T, Maruyama H, Abe T, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Int Arch Allergy Appl Immunol. 1990;92(1):64-8. doi: 10.1159/000235226.
The role of mast-cell-derived eosinophil chemotactic factor A (ECF-A) on eosinophil-rich periovular granuloma formation was examined by using mast-cell-deficient WBB6F1-W/WV mice infected with Schistosoma japonicum. The average size of granulomas formed around newly deposited eggs in the liver of W/WV mice was significantly smaller than that observed in control +/+ mice. The number of adult worms recovered or specific IgE titers were comparable between W/WV and +/+ mice. In contrast, immediate-type hypersensitivity response to specific antigen and dialyzable low-molecular weight ECF in the serum was detectable only in infected +/+ mice. When naive bone marrow eosinophils were incubated with the dialyzable fraction of infected +/+ mice sera, chemotactic reactivity of eosinophils to ECF derived from S. japonicum eggs was significantly enhanced, although that to synthetic ECF-A was depressed. Similar effects were observed when naive eosinophils were treated with synthetic ECF-A. The dialyzable fraction of infected W/WV mice sera had no such modulating effect on the chemotactic reactivity of eosinophils. These results suggest that an immediate-type hypersensitivity reaction is important in the formation of eosinophilic granulomas around S. japonicum eggs, mainly through the modulating effect of mast-cell-derived ECF-A on the chemotactic reactivity of eosinophils.
通过使用感染日本血吸虫的肥大细胞缺陷型WBB6F1-W/WV小鼠，研究了肥大细胞衍生的嗜酸性粒细胞趋化因子A（ECF-A）在富含嗜酸性粒细胞的卵周肉芽肿形成中的作用。W/WV小鼠肝脏中新沉积虫卵周围形成的肉芽肿平均大小明显小于对照 +/+ 小鼠中观察到的大小。W/WV小鼠和 +/+ 小鼠回收的成虫数量或特异性IgE滴度相当。相反，仅在感染的 +/+ 小鼠中可检测到对特定抗原和血清中可透析低分子量ECF的速发型超敏反应。当将未致敏的骨髓嗜酸性粒细胞与感染的 +/+ 小鼠血清的可透析部分一起孵育时，嗜酸性粒细胞对源自日本血吸虫卵的ECF的趋化反应性显著增强，尽管对合成ECF-A的反应性降低。当用合成ECF-A处理未致敏的嗜酸性粒细胞时，观察到类似的效果。感染的W/WV小鼠血清的可透析部分对嗜酸性粒细胞的趋化反应性没有这种调节作用。这些结果表明，速发型超敏反应在日本血吸虫卵周围嗜酸性肉芽肿的形成中很重要，主要是通过肥大细胞衍生的ECF-A对嗜酸性粒细胞趋化反应性的调节作用。
