Owhashi M, Horii Y, Ishii A, Nawa Y
Int Arch Allergy Appl Immunol. 1986;79(2):178-81. doi: 10.1159/000233967.
Eosinophil chemotactic activity was detected in the serum obtained at an acute stage of murine schistosomiasis japonica. Gel filtration of the dialyzable fraction of the serum on Sephadex G25 showed that the chemotactic component had an apparent molecular weight of less than 1,000. It was stable to heating, but was sensitive to pronase or carboxypeptidase A digestions, indicating its peptide nature. Eosinophil chemotactic activity of the dialysate of the serum from mast cell-deficient W/Wv mice infected with Schistosoma japonicum was far less than that from normal littermate +/+ mice, although the titers of specific IgE antibody to soluble egg antigen in the serum measured by passive cutaneous anaphylaxis was comparable between them. These results suggest that at least some part of low molecular weight ECF in the circulation seems to be a ECF-A derived from mast cells. Possible biological significance of circulating ECF in schistosomiasis has been discussed.
在日本血吸虫病急性期获得的小鼠血清中检测到嗜酸性粒细胞趋化活性。血清可透析部分在Sephadex G25上进行凝胶过滤显示，趋化成分的表观分子量小于1000。它对加热稳定，但对链霉蛋白酶或羧肽酶A消化敏感，表明其肽的性质。感染日本血吸虫的肥大细胞缺陷型W/Wv小鼠血清透析液的嗜酸性粒细胞趋化活性远低于正常同窝+/+小鼠，尽管通过被动皮肤过敏反应测定的血清中针对可溶性虫卵抗原的特异性IgE抗体滴度在两者之间相当。这些结果表明，循环中至少部分低分子量嗜酸性粒细胞趋化因子似乎是源自肥大细胞的嗜酸性粒细胞趋化因子A。文中讨论了循环嗜酸性粒细胞趋化因子在血吸虫病中的可能生物学意义。
