Owhashi M, Kirai N, Horii Y
Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Parasitol Res. 1997;83(1):42-6. doi: 10.1007/s004360050205.
The neutrophil is one of the sources of eosinophil chemotactic factor (ECF) in the presence of some stimulants. In the present study we showed that guinea-pig neutrophils could release ECF upon stimulation with Schistosoma japonicum eggs. ECF release from neutrophils began as early as 5 min after the stimulation and reached a peak at 20 min. When homogenate of the eggs was separated into a water-soluble fraction as soluble egg antigen (SEA) and a water-insoluble fraction (eggshell), both preparations possessed a potent neutrophil-stimulating activity to release ECF. The ECF release was dependent on the concentration of eggshells or SEA or on the number of neutrophils. The neutrophil-stimulating activity of eggshells was stable to heat, HCl, or pronase treatment but sensitive to NaOH treatment. When the eggs or eggshells were washed with acetone or Tween-20, they lost the neutrophil-stimulating activity to release ECF, indicating that the neutrophil-stimulating factor (NSF) possesses a lipid nature. The molecular weight of NSF extracted from the eggshells was estimated to be about 1000 Da by gel chromatography on Sephadex G25. The possible role of eggshells in the formation of eosinophil-rich granulomatous lesions in schistosomiasis japonica is discussed.
在某些刺激物存在的情况下，中性粒细胞是嗜酸性粒细胞趋化因子（ECF）的来源之一。在本研究中，我们发现豚鼠中性粒细胞在受到日本血吸虫卵刺激后可释放ECF。中性粒细胞释放ECF最早在刺激后5分钟开始，并在20分钟时达到峰值。当将虫卵匀浆分离成水溶性部分即可溶性虫卵抗原（SEA）和水不溶性部分（卵壳）时，这两种制剂都具有强大的刺激中性粒细胞释放ECF的活性。ECF的释放取决于卵壳或SEA的浓度或中性粒细胞的数量。卵壳刺激中性粒细胞的活性对热、盐酸或链霉蛋白酶处理稳定，但对氢氧化钠处理敏感。当用丙酮或吐温-20洗涤虫卵或卵壳时，它们失去了刺激中性粒细胞释放ECF的活性，这表明中性粒细胞刺激因子（NSF）具有脂质性质。通过在Sephadex G25上进行凝胶色谱法估计，从卵壳中提取的NSF的分子量约为1000 Da。本文讨论了卵壳在日本血吸虫病富含嗜酸性粒细胞的肉芽肿性病变形成中的可能作用。
