Department of Medical Microbiology, Medical University of Gdańsk, Poland.
Eur J Dermatol. 2011 Jan-Feb;21(1):43-6. doi: 10.1684/ejd.2010.1151.
Recurrent furunculosis (RF) caused by Staphylococcus aureus presents a difficult clinical problem and causes significant morbidity. The study aim was to characterise agr groups and detect toxin genes among S. aureus strains isolated from RF patients. Microbiological material was obtained from evacuated furuncules of 44 RF patients. Nasal swabs were obtained from both the RF patients and the controls (150 healthy volunteers with no history of RF). All strains were screened for the presence of lukS/lukF-PV, tst, sea, seb, sec, sed, eta, and etb genes. Moreover, agr specificity groups (I-IV) were identified. Antibiotic-susceptibility tests were performed by disk diffusion method and methicillin susceptibility was verified by mecA gene amplification. The investigated strains were resistant to penicillin, clindamycin, erythromycin, and tetracycline. All showed susceptibility to methicillin. Thirty-five of 44 strains tested were positive for leukocidin lukS/lukF-PV genes and 12/44 for enterotoxin seb gene. The coexistence of PVL genes and seb gene concerned 7/44 strains. The remaining toxin genes were not found. Forty-three strains belonged to agr specificity group IV including all strains with lukS/lukF-PV genes. Nasal carriage of S. aureus was observed in 27/44 (61.3%) RF patients and in 43/150 (28.6%) controls (p = 0.001). In all RF subjects, nasal strains did not differ from those isolated from furuncules in terms of lukS/lukF-PV gene status and agr specificity. To the best of our knowledge, it is the first study that shown such a predominance of agr group IV strains in RF patients.
复发性疖病（RF）由金黄色葡萄球菌引起，是一个临床难题，会导致严重的发病率。本研究旨在对金黄色葡萄球菌从 RF 患者中分离的菌株进行 agr 组特征分析，并检测其毒素基因。从 44 例 RF 患者的已排空疖中获得微生物材料。从 RF 患者和对照组（150 名无 RF 病史的健康志愿者）中均获得鼻拭子。所有菌株均进行了 lukS/lukF-PV、tst、sea、seb、sec、sed、eta 和 etb 基因的检测。此外，还确定了 agr 特异性组（I-IV）。通过纸片扩散法进行抗生素敏感性试验，通过 mecA 基因扩增验证对甲氧西林的敏感性。所研究的菌株对青霉素、克林霉素、红霉素和四环素耐药，但均对甲氧西林敏感。在 44 株检测的菌株中，35 株为白细胞毒素 lukS/lukF-PV 基因阳性，12 株为肠毒素 seb 基因阳性。有 7 株同时携带 PVL 基因和 seb 基因。其余毒素基因未检出。43 株属于 agr 特异性组 IV，包括所有携带 lukS/lukF-PV 基因的菌株。在 44 例 RF 患者中有 27 例（61.3%）和 150 例对照者中有 43 例（28.6%）携带金黄色葡萄球菌（p=0.001）。在所有 RF 患者中，鼻内分离的金黄色葡萄球菌与从疖中分离的金黄色葡萄球菌在 lukS/lukF-PV 基因状态和 agr 特异性方面没有差异。据我们所知，这是首次表明 RF 患者中存在如此高比例的 agr 组 IV 菌株的研究。
