Department of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, No 150, Haping Road, Harbin, Heilongjiang Province 150040, China.
Pathol Oncol Res. 2011 Jun;17(2):333-9. doi: 10.1007/s12253-010-9321-3. Epub 2011 Jan 14.
The signal transducer and activator of transcription-5 (STAT5) protein has been shown to play an important role in tumor progression through stimulating cell proliferation and preventing apoptosis. STAT5 activation has been observed in a variety of human tumors and cancer cell lines. However, it is not clear how activated STAT5 is expressed in colon cancer. In this study, we aimed to investigate phospho-STAT5 (activated form of STAT5) expression and its relationship with the clinicopathological factors and overall survival of patients with colonic adenocarcinoma. A total of 121 histological samples were selected for this study. Immunohistochemistry was used to detect the expression of phospho-STAT5. Analysis of the immunohistochemical staining was based on the proportion of stained cells in the field: positive, >15% stained cells, and negative, <15% stained cells. Survival times were analyzed using the Kaplan-Meier method, and the differences between groups were assessed with the log-rank test. A multivariate Cox regression model was used for prognostic power analysis. Expression of phospho-STAT5 was observed in the cytoplasms of colonic adenocarcinoma cells. Univariate analysis showed that phospho-STAT5 immunoreactivity was correlated with the depth of tumor invasion (P-value = 0.009), tumor-node-metastasis (TNM) stage (P-value = 0.048) and shorter overall survival times (P-value = 0.026). Lymph node metastasis, distant metastasis and TNM stage were associated with shorter overall survival times (P-value range from 0.003- < 0.001). Multivariate analysis showed that only distant metastasis was an independent predictor of overall survival time (P-value = 0.016). Our findings first demonstrate that phospho-STAT5 is frequently present and active in colonic adenocarcinoma and related to poor prognosis.
信号转导子和转录激活因子 5（STAT5）蛋白已被证明通过刺激细胞增殖和防止细胞凋亡在肿瘤进展中发挥重要作用。在各种人类肿瘤和癌细胞系中都观察到 STAT5 的激活。然而，尚不清楚激活的 STAT5 在结肠癌中是如何表达的。在这项研究中，我们旨在研究磷酸化 STAT5（STAT5 的激活形式）的表达及其与结肠腺癌患者的临床病理因素和总生存时间的关系。本研究共选择了 121 个组织学样本。免疫组织化学用于检测磷酸化 STAT5 的表达。基于染色细胞在视野中的比例对免疫组织化学染色进行分析：阳性，>15%染色细胞；阴性，<15%染色细胞。使用 Kaplan-Meier 方法分析生存时间，使用对数秩检验评估组间差异。使用多变量 Cox 回归模型进行预后能力分析。在结肠腺癌细胞的细胞质中观察到磷酸化 STAT5 的表达。单因素分析显示，磷酸化 STAT5 免疫反应性与肿瘤浸润深度（P 值=0.009）、肿瘤-淋巴结-转移（TNM）分期（P 值=0.048）和较短的总生存时间相关（P 值=0.026）。淋巴结转移、远处转移和 TNM 分期与较短的总生存时间相关（P 值范围从 0.003 到<0.001）。多因素分析显示，只有远处转移是总生存时间的独立预测因子（P 值=0.016）。我们的研究结果首次表明，磷酸化 STAT5 在结肠腺癌中频繁存在且活跃，并与不良预后相关。
