Liu Zhaoxiu, Guan Chengqi, Lu Cuihua, Liu Yanmei, Ni Runzhou, Xiao Mingbing, Bian Zhaolian
Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
Chinese Evidence-Based Medicine Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610000, China.
Pathol Res Pract. 2018 Jul;214(7):968-973. doi: 10.1016/j.prp.2018.05.017. Epub 2018 May 22.
Nucleolar and spindle-associated protein 1 (NUSAP1) is an indispensable mitotic regulator. Aberrant NUSAP1 expression is associated with perturbed mitosis and tumorigenesis. In this study, we investigated the clinical significance of NUSAP1 expression in colon cancer.
Immunohistochemical staining was performed to determine NUSAP1 protein levels in paraffin colon tumor specimens. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was conducted to detect NUSAP1 mRNA levels in colon tumor samples. The association between NUSAP1 protein expression and clinicopathological characteristics of patients with colon cancer was assessed. A Kaplan-Meier analysis was performed to determine the prognostic significance of NUSAP1 in colon cancer. A Cox proportional hazards model was used to calculate univariate and multivariate hazard ratios for the NUSAP1 and other clinicopathological variables.
NUSAP1 protein and mRNA levels were significantly higher in colon tumor tissues than in paired non-cancerous adjacent tissues (P < 0.001, respectively). NUSAP1 protein expression was significantly correlated with histopathological grading (P < 0.001), depth of invasion (P = 0.001), lymph node metastasis (P < 0.001) and TNM stage (P < 0.001). The overall survival rate of patients with high NUSAP1 expression was significantly lower than for patients with low NUSAP1 expression (log-rank test, P < 0.001). A multivariate Cox model demonstrated that NUSAP1 is an independent risk factor for overall survival (P = 0.025).
NUSAP1 is overexpressed in colon cancer and high expression of NUSAP1 acts as an independent predictive factor for poor prognosis in colon cancer.
核仁与纺锤体相关蛋白1（NUSAP1）是一种不可或缺的有丝分裂调节因子。NUSAP1表达异常与有丝分裂紊乱和肿瘤发生相关。在本研究中，我们调查了NUSAP1表达在结肠癌中的临床意义。
采用免疫组织化学染色法测定石蜡包埋的结肠肿瘤标本中NUSAP1蛋白水平。进行实时荧光定量聚合酶链反应（RT-qPCR）以检测结肠肿瘤样本中NUSAP1 mRNA水平。评估NUSAP1蛋白表达与结肠癌患者临床病理特征之间的关联。进行Kaplan-Meier分析以确定NUSAP1在结肠癌中的预后意义。使用Cox比例风险模型计算NUSAP1及其他临床病理变量的单变量和多变量风险比。
结肠肿瘤组织中NUSAP1蛋白和mRNA水平显著高于配对的癌旁非肿瘤组织（P值分别<0.001）。NUSAP1蛋白表达与组织病理学分级（P<0.001）、浸润深度（P=0.001）、淋巴结转移（P<0.001）和TNM分期（P<0.001）显著相关。NUSAP1高表达患者的总生存率显著低于NUSAP1低表达患者（对数秩检验，P<0.001）。多变量Cox模型表明NUSAP1是总生存的独立危险因素（P=0.025）。
NUSAP1在结肠癌中过表达，NUSAP1的高表达是结肠癌预后不良的独立预测因素。
