Maeda K, Feyles V, McGarry R C, Jerry L M
Oncology Research Group, University of Calgary, Alberta, Canada.
J Invest Dermatol. 1990 Dec;95(6):665-70. doi: 10.1111/1523-1747.ep12514336.
Five human neuroblastoma cell lines were examined for expression of a human melanosome-associated antigen (HMSA). Only cell line SK-N-SH reacted with a monoclonal antibody, HMSA-2, shown to recognize melanosomal glycoproteins. To further characterize the melanocytic lineages of SK-N-SH, three morphologically distinct clones designated SK-N-SH-N (neuroblast type), SK-N-SH-F (fibroblast type), and SK-N-SH-EP (epithelial type) were established by colony formation cloning. By fluorescence-activated cell sorter analysis and tyrosinase assay, we found that only SK-N-SH-EP and SK-N-SH-F reacted with HMSA-2 and had tyrosinase activity. These results suggest that epithelial-type and fibroblast-type cells appear to possess the melanocytic potential, but not neuroblast-type cells. Furthermore, SK-N-SH-EP was found to spontaneously convert to neuroblast-type or fibroblast-type cells, whereas SK-N-SH-N and SK-N-SH-F clones have remained morphologically stable. Our results suggest that at least one neuroblastoma cell line, SK-N-SH, may be an excellent model for investigating clonal maturation and the melanocytic differentiation of neuroblastoma.
对五个人类神经母细胞瘤细胞系进行了人类黑素小体相关抗原（HMSA）表达检测。只有细胞系SK-N-SH与一种单克隆抗体HMSA-2发生反应，该抗体已证实可识别黑素小体糖蛋白。为进一步表征SK-N-SH的黑素细胞谱系，通过集落形成克隆建立了三个形态上不同的克隆，分别命名为SK-N-SH-N（神经母细胞型）、SK-N-SH-F（成纤维细胞型）和SK-N-SH-EP（上皮细胞型）。通过荧光激活细胞分选分析和酪氨酸酶测定，我们发现只有SK-N-SH-EP和SK-N-SH-F与HMSA-2发生反应并具有酪氨酸酶活性。这些结果表明上皮细胞型和成纤维细胞型细胞似乎具有黑素细胞潜能，而神经母细胞型细胞则没有。此外，发现SK-N-SH-EP可自发转化为神经母细胞型或成纤维细胞型细胞，而SK-N-SH-N和SK-N-SH-F克隆在形态上保持稳定。我们的结果表明，至少一种神经母细胞瘤细胞系SK-N-SH可能是研究神经母细胞瘤克隆成熟和黑素细胞分化的理想模型。
