Wennink J M, Delemarre-van de Waal H A, Schoemaker R, Schoemaker H, Schoemaker J
Department of Pediatrics, Academic Hospital, Vrije Universiteit, Amsterdam, The Netherlands.
Clin Endocrinol (Oxf). 1990 Sep;33(3):333-44. doi: 10.1111/j.1365-2265.1990.tb00498.x.
Pulsatile gonadotrophin secretion patterns were studied in 36 healthy girls by measuring every 10 min and applying immunoradiometric assays (IRMA). Different stages of puberty were associated with significant changes in the plasma LH and FSH levels, pulse numbers (Pno) and pulse amplitudes (pA). Plasma LH was not detectable by day or night in young prepubertal girls (B1), neither was plasma oestradiol (E2); however, plasma FSH was detectable in a pulsatile pattern. In the older prepubertal girls (B1-onset) a discrete pulsatile LH pattern became detectable only during the night; plasma FSH tended to rise, while E2 became just detectable. In the early pubertal girls (B2) most daytime LH values were above the detection limit, in some with low-amplitude pulses. At night, pulses with a wide range of pulse amplitudes were detected. Plasma FSH increased further, plasma E2 only slightly. With the progression of puberty the plasma LH and FSH levels, Pno and pA increased significantly from stage B2 to B3 during the day (P less than or equal to 0.05) and close to significance during the night (0.05 less than or equal to P less than or equal to 0.1). However, in stage B4 the secretory characteristics tended to decline, while from stage B3 onwards plasma E2 started to rise rapidly (P less than or equal to 0.05, during the night from stage B2 to B3, during the day from B3 to B4m-). Simultaneous LH and FSH pulses were observed throughout puberty, usually during the night. Using these IRMA methods nocturnal LH in older prepubertal girls and both diurnal and nocturnal FSH pulsatility could be demonstrated in young prepubertal girls. From this study we conclude that (1) puberty in girls, as in boys, may be brought about by an increasing GnRH secretion both in frequency and amplitude, first appearing during the night. This increased GnRH stimulation results in LH secretion only during the night; (2) a cyclical pulsatile LH pattern including an LH surge can be established before the menarche; the capacity for positive feedback activity is not the final maturation characteristic to achieve an ovulatory menstrual cycle.
通过每10分钟测量一次并应用免疫放射测定法（IRMA），对36名健康女孩的促性腺激素脉冲分泌模式进行了研究。青春期的不同阶段与血浆促黄体生成素（LH）和促卵泡生成素（FSH）水平、脉冲数（Pno）和脉冲幅度（pA）的显著变化相关。青春期前的年轻女孩（B1期）无论白天还是夜晚，血浆LH均无法检测到，血浆雌二醇（E2）也无法检测到；然而，血浆FSH以脉冲模式可检测到。在年龄较大的青春期前女孩（B1期 - 青春期开始）中，仅在夜间可检测到离散的LH脉冲模式；血浆FSH趋于升高，而E2刚刚可检测到。在青春期早期女孩（B2期）中，大多数白天的LH值高于检测限，有些呈低幅度脉冲。在夜间，检测到幅度范围广泛的脉冲。血浆FSH进一步升高，血浆E2仅略有升高。随着青春期的进展，白天从B2期到B3期，血浆LH和FSH水平、Pno和pA显著升高（P≤0.05），夜间接近显著水平（0.05≤P≤0.1）。然而，在B4期，分泌特征趋于下降，而从B3期开始血浆E2开始迅速升高（夜间从B2期到B3期、白天从B3期到B4期，P≤0.05）。在整个青春期都观察到LH和FSH同时出现脉冲，通常在夜间。使用这些IRMA方法，可以证明年龄较大的青春期前女孩夜间的LH以及青春期前年轻女孩白天和夜间的FSH脉冲性。从这项研究中我们得出结论：（1）女孩的青春期，与男孩一样，可能是由GnRH分泌在频率和幅度上的增加引起的，首先出现在夜间。这种GnRH刺激的增加仅在夜间导致LH分泌；（2）在初潮前可以建立包括LH峰的周期性LH脉冲模式；正反馈活动的能力不是实现排卵月经周期的最终成熟特征。
