Salata R A, Martinez-Palomo A, Canales L, Murray H W, Trevino N, Ravdin J I
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Infect Immun. 1990 Dec;58(12):3941-6. doi: 10.1128/iai.58.12.3941-3946.1990.
Lymphocytes from patients cured of amebic liver abscesses proliferate and produce gamma interferon upon incubation with soluble Entamoeba histolytica antigen: however, amebic liver abscesses exhibit a relentless progression without treatment. To determine whether suppressive factors are present in sera, we studied T-lymphocyte responses to total soluble E. histolytica antigen by using cells from five patients treated for amebic liver abscesses in the presence of 15 different immune sera and 10 control sera. In the presence of immune sera, E. histolytica antigen-induced lymphocyte proliferation decreased by 63% and production of gamma interferon was reduced by 93.2% (P less than 0.01). Immune sera had no effect on the mitogenic responses of patient lymphocytes to phytohemagglutinin or on the proliferative responses of control lymphocytes to phytohemagglutinin or tetanus toxoid. The suppressive activity of immune sera diminished as the time between therapy for amebic liver abscesses and serum collection increased (P less than 0.05). Suppressive activity did not correlate with the titers of serum anti-amebic antibody and was not affected when serum was absorbed with viable amebic trophozoites. In conclusion, soluble factors present in the sera of amebic liver abscess patients suppressed in vitro lymphocyte responses to E. histolytica antigen and may have contributed to the lack of development of effective in vivo cell-mediated immune responses following the onset of amebic liver abscesses.
来自已治愈阿米巴肝脓肿患者的淋巴细胞在与可溶性溶组织内阿米巴抗原孵育时会增殖并产生γ干扰素：然而，未经治疗的阿米巴肝脓肿会持续进展。为了确定血清中是否存在抑制因子，我们使用了来自5名接受阿米巴肝脓肿治疗患者的细胞，在15种不同的免疫血清和10种对照血清存在的情况下，研究了T淋巴细胞对总可溶性溶组织内阿米巴抗原的反应。在免疫血清存在的情况下，溶组织内阿米巴抗原诱导的淋巴细胞增殖减少了63%，γ干扰素的产生减少了93.2%（P<0.01）。免疫血清对患者淋巴细胞对植物血凝素的促有丝分裂反应或对照淋巴细胞对植物血凝素或破伤风类毒素的增殖反应没有影响。免疫血清的抑制活性随着阿米巴肝脓肿治疗与血清采集之间时间的增加而降低（P<0.05）。抑制活性与血清抗阿米巴抗体的滴度无关，并且当血清用活的阿米巴滋养体吸收时不受影响。总之，阿米巴肝脓肿患者血清中存在的可溶性因子抑制了体外淋巴细胞对溶组织内阿米巴抗原的反应，并且可能导致了阿米巴肝脓肿发病后体内有效细胞介导免疫反应的缺乏。
