Faculty of Chemistry, Northeast Normal University, Changchun, Jilin, PR China.
Talanta. 2011 Feb 15;83(5):1386-91. doi: 10.1016/j.talanta.2010.11.022. Epub 2010 Nov 18.
Ordered mesoporous carbon-Au nanoparticles (OMC-Au) nanocomposites were synthesized by a one-step chemical reduction route. Due to the large surface area and high conductivity of OMC, good biocompatibility of OMC and Au nanoparticles, a mediator-free glucose biosensor was fabricated by immobilizing glucose oxidase (GOD) on the OMC-Au nanocomposites modified glassy carbon (GC) electrode. Direct electron transfer between GOD and the electrode was achieved and the electron transfer rate constant (k(s)) was calculated to be 5.03 s(-1). The Michalis-Menten constant (K(M)(app)) value of GOD immobilized on the OMC-Au/GC electrode surface was found to be 0.6 mM. The glucose biosensor exhibits a linear range from 0.05 to 20.0 mM. This biosensor also shows good reproducibility, excellent stability and the negligible interferences from ascorbic acid and uric acid.
有序介孔碳-金纳米粒子（OMC-Au）复合材料通过一步化学还原法合成。由于 OMC 的比表面积大、导电性好、OMC 和 Au 纳米粒子的生物相容性好，因此通过将葡萄糖氧化酶（GOD）固定在 OMC-Au 纳米复合材料修饰的玻碳（GC）电极上，制备了一种无介体的葡萄糖生物传感器。GOD 与电极之间实现了直接电子转移，并且计算了电子转移速率常数（k(s)）为 5.03 s(-1)。在 OMC-Au/GC 电极表面固定的 GOD 的迈克尔米门常数（K(M)(app)）值为 0.6 mM。葡萄糖生物传感器的线性范围为 0.05 至 20.0 mM。该生物传感器还表现出良好的重现性、优异的稳定性和可忽略的抗坏血酸和尿酸干扰。
