Paul S K, Hossain M A, Mahmud M C, Ahmed S, Hossain M A, Nandi A K, Tabassum S
Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh.
Mymensingh Med J. 2011 Jan;20(1):1-8.
A total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (Dharmapasha health complex, Sunamgonj) in Bangladesh from August 2004 to May 2006 were tested for rotavirus by Polyacrylamide Gel Electrophoresis (PAGE). The diversity of rotavirus was investigated using electropherotyping and reverse transcription-PCR amplification of the VP7 and VP4 genes. The electrophoretic patterns of dsRNA of rotavirus showed 9 different migrations (6 long and 3 short) by PAGE. In the year 2004-2006, group A rotavirus was detected in 112 out of 280(40.0%) specimens. G and P genotyping was performed among the 46 representative positive specimens, 20(43.5%) were emerging strain G9P[8], which were associated with VP6 genotype II (subgroup II), and NSP4 genotype B, followed by 16(34.8%) G2P[4], 8(17.4%) G1P[8] and 2(4.4%) G4P[8] strains. G9P[8] was found to be the most predominant strain in 2004, but the prevalence rate abruptly decreased during the period 2005-2006. In addition G2P[4] was the most prevalent strain in 2005 and 2006. G1P[8] was less prevalent in the study period then the previous years. Nucleotide Sequence identity of VP7 gene of G9 rotaviruses were higher than 99.4% with each other and all the G9 rotavirus strains in this study clustered in a single branch of the phylogenetic tree. Nucleotide sequence identity of complete VP4 gene of P[8] rotaviruses were more than 99.7% with each other and all the P[8] rotavirus strains in this study grouped in a single cluster suggesting recent emergence from a common ancestor. An important finding of this study is that the genetic profile of rotavirus is changing within very short period in Bangladesh and continued surveillance of the circulating strains is necessary to detect new strains or new variants which can escape immune protection induced by available vaccines.
2004年8月至2006年5月期间，从孟加拉国一家乡村医院（苏纳甘杰的达马帕夏健康中心）的急性肠胃炎患者中收集了280份粪便样本，采用聚丙烯酰胺凝胶电泳（PAGE）法检测轮状病毒。通过电泳分型以及VP7和VP4基因的逆转录-聚合酶链反应扩增来研究轮状病毒的多样性。轮状病毒双链RNA的电泳图谱通过PAGE显示出9种不同的迁移情况（6种长型和3种短型）。在2004年至2006年期间,280份样本中有112份（40.0%）检测出A组轮状病毒。对46份具有代表性的阳性样本进行了G和P基因分型，其中20份（43.5%）为新出现的G9P[8]毒株，与VP6基因型II（亚组II）以及NSP4基因型B相关，其次是16份（34.8%）G2P[4]、8份（17.4%）G1P[8]和2份（4.4%）G4P[8]毒株。G9P[8]在2004年是最主要的毒株，但在2005年至2006年期间流行率急剧下降。此外，G2P[4]在2005年和2006年是最流行的毒株。G1P[8]在研究期间的流行率低于前几年。G9轮状病毒VP7基因的核苷酸序列同一性相互之间高于99.4%，并且本研究中的所有G9轮状病毒毒株都聚集在系统发育树的一个单分支中。P[8]轮状病毒完整VP4基因的核苷酸序列同一性相互之间超过99.7%，并且本研究中的所有P[8]轮状病毒毒株都聚集在一个单簇中，表明它们最近源自一个共同祖先。本研究的一个重要发现是，在孟加拉国，轮状病毒的基因谱在很短时间内正在发生变化，持续监测流行毒株对于检测可能逃避现有疫苗诱导的免疫保护的新毒株或新变种是必要的。
