Sultana T, Sultana T, Rahman M Q, Rahman F, Islam M S, Ahmed A N
Dhaka Medical College Hospital, Dhaka, Bangladesh.
Mymensingh Med J. 2011 Jan;20(1):71-7.
The morphology of red cells by phase-contrast microscopy (PCM) is a useful diagnostic marker for glomerular haematuria. This study evaluated the value of urinary dysmorphic red cells and G1 cells (special type of dysmorphic red cell) count by PCM providing a simple, cost effective and low risk technique in the diagnosis of glomerular diseases. Urine samples of 120 patients with haematuria and proteinuria were examined and the percentage of dysmorphic red cells and G1 cells were calculated. Cases were divided into two groups; group I (>20% dysmorphic red cells- glomerular group) and group II (≤20% dysmorphic red cells as non glomerular group). Renal histopathology was used as the gold standard method for the diagnosis of glomerulonephritis. Results from PCM showed a sensitivity of 92.7%, specificity 100% by the detection of dysmorphic red cell while by the detection of G1 cells, a sensitivity of 97.6% and specificity 100% were observed. The percentage of G1 cells is superior to counting dysmorphic red cells. The high sensitivity of phase contrast microscopy confirms its usefulness for the detection of dysmorphic red cells and G1 cells that can guide clinicians in the identification of the site of haematuria using non invasive tests.
通过相差显微镜（PCM）观察红细胞形态是诊断肾小球性血尿的一项有用的诊断标志物。本研究评估了通过PCM检测尿中异形红细胞和G1细胞（一种特殊类型的异形红细胞）数量的价值，该方法提供了一种简单、经济且风险低的技术用于诊断肾小球疾病。对120例血尿和蛋白尿患者的尿液样本进行检测，并计算异形红细胞和G1细胞的百分比。病例分为两组：第一组（异形红细胞>20%——肾小球组）和第二组（异形红细胞≤20%——非肾小球组）。肾组织病理学被用作诊断肾小球肾炎的金标准方法。PCM检测结果显示，检测异形红细胞时灵敏度为92.7%，特异性为100%；而检测G1细胞时，灵敏度为97.6%，特异性为100%。G1细胞的百分比优于计数异形红细胞。相差显微镜的高灵敏度证实了其在检测异形红细胞和G1细胞方面的有用性，这可以指导临床医生使用非侵入性检测来确定血尿的部位。
