Lettgen B, Wohlmuth A
Department of Paediatrics, University of Essen, Germany.
Pediatr Nephrol. 1995 Aug;9(4):435-7. doi: 10.1007/BF00866720.
Urine samples from 100 children and adolescents with micro- or macrohaematuria were investigated using phase contrast microscopy to establish the percentage of G1-cells that could differentiate glomerular from non-glomerular haematuria. The G1-cell is a special form of dysmorphic erythrocyte which seems to be specific for glomerular haematuria. Glomerular haematuria, defined by clinical criteria from biopsy, physical examination, standard laboratory evaluation and family history, was observed in 51 patients (group 1). Non-glomerular haematuria was found in 49 patients (group 2). The latter group had urinary tract infections, urolithiasis, hypercalciuria or haematuria caused by urological operation or diagnostic procedure. The percentage of dysmorphic erythrocytes differed significantly between the two groups studied (42 +/- 3% in group 1 vs. 6 +/- 1% in group 2, mean +/- SEM, P < 0.01); there was also a significant difference in G1-cells (19.4 +/- 1.7% in group 1 vs. 0.6 +/- 0.2% in group 2, mean +/- SEM, P < 0.01). When glomerular haematuria was defined on the basis of > or = 30% dysmorphic erythrocytes by phase contrast microscopy, sensitivity, specificity and efficiency were 71%, 100% and 85%, respectively. When glomerular haematuria was defined on the basis of > or = 5% G1-cells, sensitivity, specificity and efficiency were 100%, 100% and 100%, respectively. The differentiation of glomerular and non-glomerular haematuria in children by determination of G1-cells appears to be more sensitive and efficient than the determination of the percentage of dysmorphic erythrocytes by phase contrast microscopy.
对100名患有镜下或肉眼血尿的儿童及青少年的尿液样本进行相差显微镜检查，以确定能区分肾小球性血尿与非肾小球性血尿的G1细胞百分比。G1细胞是一种特殊形态的异形红细胞，似乎是肾小球性血尿所特有的。根据活检、体格检查、标准实验室评估及家族史的临床标准，51例患者（第1组）被诊断为肾小球性血尿。49例患者（第2组）被诊断为非肾小球性血尿。后一组患者患有尿路感染、尿路结石、高钙尿症或由泌尿外科手术或诊断程序导致的血尿。研究的两组异形红细胞百分比存在显著差异（第1组为42±3%，第2组为6±1%，均值±标准误，P<0.01）；G1细胞也存在显著差异（第1组为19.4±1.7%，第2组为0.6±0.2%，均值±标准误，P<0.01）。当通过相差显微镜将肾小球性血尿定义为异形红细胞≥30%时，敏感性、特异性和效率分别为71%、100%和85%。当将肾小球性血尿定义为G1细胞≥5%时，敏感性、特异性和效率分别为100%、100%和100%。通过测定G1细胞来区分儿童的肾小球性血尿和非肾小球性血尿，似乎比通过相差显微镜测定异形红细胞百分比更敏感、更有效。
