Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA.
Nat Methods. 2011 Feb;8(2):135-8. doi: 10.1038/nmeth.1556. Epub 2011 Jan 16.
Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.
鉴定调控脂肪积累的基因对于基础和医学研究很重要；在广泛使用的模式生物秀丽隐杆线虫中，对这些基因进行遗传筛选需要对脂类进行体内定量。我们通过无标记的受激拉曼散射（SRS）显微镜对脂质进行定量成像，展示了基于 RNA 干扰的筛选，克服了相干反斯托克斯拉曼散射显微镜的主要限制。我们的筛选得到了八个新的脂肪储存遗传调控因子。
