Liu Zhenglong, Li Xia, Ge Qinlan, Ding Mei, Huang Xun
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
J Genet Genomics. 2014 May 20;41(5):305-13. doi: 10.1016/j.jgg.2014.03.002. Epub 2014 Mar 19.
Fat storage disorders including obesity are pandemic human health problems. As a genetically amenable model organism, Caenorhabditis elegans has often been used to explore the molecular mechanisms of fat storage regulation. Dye staining of fixed animals and stimulated Raman scattering (SRS) microscopy methods have been used successfully to study fat storage, but a genetic screening system that takes full advantage of C. elegans transparency to perform live imaging of fluorescent protein reporters has not yet been reported. Here, we investigated the tissue-specific expression of the GFP fusion of Perilipin 1 (PLIN1), a Drosophila lipid droplet-associated protein, in C. elegans. Our results indicate that PLIN1::GFP labels lipid droplets and can be used as a fat storage indicator in live worms. Through an RNAi screen, we further identified several previously uncharacterized new fat storage regulators.
包括肥胖症在内的脂肪储存障碍是全球性的人类健康问题。秀丽隐杆线虫作为一种易于进行基因操作的模式生物，常被用于探索脂肪储存调控的分子机制。固定动物的染料染色和受激拉曼散射（SRS）显微镜方法已成功用于研究脂肪储存，但尚未报道一种能充分利用秀丽隐杆线虫的透明性对荧光蛋白报告基因进行活体成像的遗传筛选系统。在此，我们研究了果蝇脂滴相关蛋白 perilipin 1（PLIN1）的 GFP 融合蛋白在秀丽隐杆线虫中的组织特异性表达。我们的结果表明，PLIN1::GFP 标记脂滴，可作为活线虫中脂肪储存的指标。通过 RNAi 筛选，我们进一步鉴定出了几种以前未被表征的新的脂肪储存调节因子。
