无标记脂质动力学成像：相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS）显微镜技术。
Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.
机构信息
Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Curr Opin Genet Dev. 2011 Oct;21(5):585-90. doi: 10.1016/j.gde.2011.09.003. Epub 2011 Sep 22.
Abstract
The recently developed Coherent Anti-stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy have provided new methods to visualize the localization and regulation of biological molecules without the use of invasive and potentially perturbative labels. They allow rapid imaging of specific molecules with high resolution and sensitivity. These tools have been effectively applied to the study of lipid metabolism using Caenorhabditis elegans as a genetic model, unraveling new lipid storage phenotypes and their regulatory mechanisms. Here we review the underlying principle of CARS and SRS microscopy, as well as their recent applications in lipid biology research in C. elegans.
摘要
最近发展起来的相干反斯托克斯拉曼散射（CARS）显微镜和受激拉曼散射（SRS）显微镜为可视化生物分子的定位和调控提供了新的方法，无需使用侵入性和潜在的干扰性标记物。它们可以快速高分辨率和高灵敏度地成像特定分子。这些工具已成功应用于使用秀丽隐杆线虫作为遗传模型的脂质代谢研究，揭示了新的脂质储存表型及其调控机制。在这里，我们回顾了 CARS 和 SRS 显微镜的基本原理，以及它们在秀丽隐杆线虫脂质生物学研究中的最新应用。
相似文献
1
Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.无标记脂质动力学成像：相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS）显微镜技术。
Curr Opin Genet Dev. 2011 Oct;21(5):585-90. doi: 10.1016/j.gde.2011.09.003. Epub 2011 Sep 22.
2
Shedding new light on lipid functions with CARS and SRS microscopy.利用相干反斯托克斯拉曼散射（CARS）显微镜和受激拉曼散射（SRS）显微镜揭示脂质功能的新见解。
Biochim Biophys Acta. 2014 Aug;1841(8):1120-9. doi: 10.1016/j.bbalip.2014.02.003. Epub 2014 Feb 25.
3
Label-free biomedical imaging of lipids by stimulated Raman scattering microscopy.利用受激拉曼散射显微镜对脂质进行无标记生物医学成像。
Curr Protoc Mol Biol. 2015 Jan 5;109:30.3.1-30.3.17. doi: 10.1002/0471142727.mb3003s109.
4
Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy.利用相干反斯托克斯拉曼散射（CARS）显微镜监测秀丽隐杆线虫中的脂质储存情况。
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14658-63. doi: 10.1073/pnas.0703594104. Epub 2007 Sep 5.
5
Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy.通过受激拉曼散射显微镜对单细胞和组织进行快速振动成像。
Acc Chem Res. 2014 Aug 19;47(8):2282-90. doi: 10.1021/ar400331q. Epub 2014 May 28.
6
RNAi screening for fat regulatory genes with SRS microscopy.利用 SRS 显微镜进行脂肪调节基因的 RNAi 筛选。
Nat Methods. 2011 Feb;8(2):135-8. doi: 10.1038/nmeth.1556. Epub 2011 Jan 16.
7
Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy.相干反斯托克斯拉曼散射显微镜技术为脂质生物学研究带来新曙光。
J Lipid Res. 2010 Nov;51(11):3091-102. doi: 10.1194/jlr.R008730. Epub 2010 Aug 16.
8
Molecular orientational order probed by coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy: a spectral comparative study.通过相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS）显微镜探测分子取向有序性：光谱比较研究。
J Phys Chem B. 2015 Feb 19;119(7):3242-9. doi: 10.1021/jp5113813. Epub 2015 Jan 30.
9
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging.高光谱受激拉曼散射与相干反斯托克斯拉曼散射显微镜的化学成像直接比较。
J Vis Exp. 2022 Apr 28(182). doi: 10.3791/63677.
10
Applications of coherent Raman scattering microscopies to clinical and biological studies.相干拉曼散射显微镜技术在临床和生物学研究中的应用。
Analyst. 2015 Jun 21;140(12):3897-909. doi: 10.1039/c5an00178a. Epub 2015 Mar 26.
引用本文的文献
1
Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.受激拉曼散射显微镜原理：在高时空极限下崭露头角。
J Phys Chem C Nanomater Interfaces. 2025 Mar 27;129(12):5789-5797. doi: 10.1021/acs.jpcc.5c00655. Epub 2025 Mar 13.
2
Current Trends and Advances in Nanoplatforms-Based Imaging for Cancer Diagnosis.基于纳米平台的癌症诊断成像的当前趋势与进展
Indian J Microbiol. 2025 Mar;65(1):137-176. doi: 10.1007/s12088-024-01373-9. Epub 2024 Aug 9.
3
Raman analysis of lipids in cells: Current applications and future prospects.细胞中脂质的拉曼分析：当前应用与未来前景
J Pharm Anal. 2025 Apr;15(4):101136. doi: 10.1016/j.jpha.2024.101136. Epub 2024 Nov 1.
4
SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in .受激拉曼散射显微镜鉴定出卵黄生成的抑制是热量限制延长秀丽隐杆线虫寿命的一个介导因素。 （注：原文中“in ”后面缺少具体物种信息，这里补充了“秀丽隐杆线虫”使句子完整通顺，你可根据实际情况修改。）
bioRxiv. 2025 Feb 3:2025.01.31.636008. doi: 10.1101/2025.01.31.636008.
5
Discussion on the comparison of Raman spectroscopy and cardiovascular disease-related imaging techniques and the future applications of Raman technology: a systematic review.拉曼光谱与心血管疾病相关成像技术的比较及拉曼技术未来应用的讨论：一项系统综述
Lasers Med Sci. 2025 Feb 24;40(1):116. doi: 10.1007/s10103-025-04315-z.
6
Lipid metabolism, remodelling and intercellular transfer in the CNS.中枢神经系统中的脂质代谢、重塑及细胞间转运
Nat Rev Neurosci. 2025 Apr;26(4):214-231. doi: 10.1038/s41583-025-00908-3. Epub 2025 Feb 19.
7
Raman cell sorting for single-cell research.用于单细胞研究的拉曼细胞分选
Front Bioeng Biotechnol. 2024 May 20;12:1389143. doi: 10.3389/fbioe.2024.1389143. eCollection 2024.
8
Viewing life without labels under optical microscopes.在光学显微镜下观察无标签的生命。
Commun Biol. 2023 May 25;6(1):559. doi: 10.1038/s42003-023-04934-8.
9
Label-Free Optical Metabolic Imaging in Cells and Tissues.无标记光学代谢成像在细胞和组织中的应用。
Annu Rev Biomed Eng. 2023 Jun 8;25:413-443. doi: 10.1146/annurev-bioeng-071516-044730. Epub 2023 Apr 27.
10
Application of in Lipid Metabolism Research.在脂质代谢研究中的应用。
Int J Mol Sci. 2023 Jan 7;24(2):1173. doi: 10.3390/ijms24021173.
本文引用的文献
1
Highly specific label-free molecular imaging with spectrally tailored excitation stimulated Raman scattering (STE-SRS) microscopy.利用光谱定制激发受激拉曼散射（STE-SRS）显微镜进行的高特异性无标记分子成像。
Nat Photonics. 2011;5(2):103-109. doi: 10.1038/nphoton.2010.294. Epub 2011 Jan 16.
2
Coherent nonlinear optical imaging: beyond fluorescence microscopy.相干非线性光学成像：超越荧光显微镜
Annu Rev Phys Chem. 2011;62:507-30. doi: 10.1146/annurev.physchem.012809.103512.
3
RNAi screening for fat regulatory genes with SRS microscopy.利用 SRS 显微镜进行脂肪调节基因的 RNAi 筛选。
Nat Methods. 2011 Feb;8(2):135-8. doi: 10.1038/nmeth.1556. Epub 2011 Jan 16.
4
Lipid droplets as ubiquitous fat storage organelles in C. elegans.脂滴是秀丽隐杆线虫中普遍存在的脂肪储存细胞器。
BMC Cell Biol. 2010 Dec 8;11:96. doi: 10.1186/1471-2121-11-96.
5
Video-rate molecular imaging in vivo with stimulated Raman scattering.利用受激拉曼散射进行体内视频速率分子成像。
Science. 2010 Dec 3;330(6009):1368-70. doi: 10.1126/science.1197236.
6
A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods.使用标记和非标记方法对线虫秀丽隐杆线虫中脂肪储存定量的比较研究。
PLoS One. 2010 Sep 16;5(9):e12810. doi: 10.1371/journal.pone.0012810.
7
Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine.相干反斯托克斯拉曼散射显微镜：生物学和医学中的化学成像。
Annu Rev Anal Chem (Palo Alto Calif). 2008;1:883-909. doi: 10.1146/annurev.anchem.1.031207.112754.
8
Imaging growth of neurites in conditioned hydrogel by coherent anti-stokes raman scattering microscopy.通过相干反斯托克斯拉曼散射显微镜观察条件化水凝胶中的神经突生长。
Organogenesis. 2009 Oct;5(4):231-7. doi: 10.4161/org.5.4.10404.
9
Lipofuscin: detection and quantification by microscopic techniques.脂褐素：通过显微镜技术进行检测和定量分析。
Methods Mol Biol. 2010;594:173-93. doi: 10.1007/978-1-60761-411-1_13.
10
C. elegans major fats are stored in vesicles distinct from lysosome-related organelles.秀丽隐杆线虫的主要脂肪储存在与溶酶体相关细胞器不同的小泡中。
Cell Metab. 2009 Nov;10(5):430-5. doi: 10.1016/j.cmet.2009.10.002.
Zotero 插件