Department of Neurosurgery, Tangdu Hospital, the Forth Military Medical University, Xi'an, China.
J Surg Oncol. 2011 Jun;103(8):813-7. doi: 10.1002/jso.21851. Epub 2011 Jan 16.
Notch signaling plays a complex role in human malignancy. It can affect cell proliferation, differentiation, and apoptosis either positively or negatively, depending on cell type.
Clinical gliomas from 274 patients who had not received chemotherapy were collected, and the expression of Notch1 protein was assessed by immunochemistry assays. Staining evaluation results were analyzed statistically in relation to various clinicopathological characters. Kaplan-Meier and Cox proportional hazards regression methods were used to assess associations of Notch1 expression with glioma survival.
We found that the levels of expression of Notch1 protein were higher in glioma than control normal brain tissue. Moreover, the expression of Notch1 was associated closely with glioma progression, since expression levels increased from grades I to IV glioma. The overall survival rate was significantly lower for patients with Notch1 positive than those with Notch1 negative tumors. Multivariate analysis showed that Notch1 was an independent prognostic factor for patients with glioma.
These findings we confirm that Notch1 expression is up-regulated in glioma and related to tumor progression. Notch1 might also serve as a novel prognostic marker that is independent of, and additive to, the WHO grade.
Notch 信号通路在人类恶性肿瘤中发挥着复杂的作用。其对细胞增殖、分化和凋亡的影响可以是正向的，也可以是负向的，这取决于细胞类型。
收集了 274 例未经化疗的临床脑胶质瘤患者的样本，通过免疫组织化学方法评估 Notch1 蛋白的表达情况。对染色评估结果进行统计学分析，以探讨 Notch1 表达与胶质瘤临床病理特征的关系。采用 Kaplan-Meier 法和 Cox 比例风险回归方法评估 Notch1 表达与胶质瘤患者生存的关系。
我们发现 Notch1 蛋白在脑胶质瘤中的表达水平高于正常脑组织。此外，Notch1 的表达与胶质瘤的进展密切相关，因为 Notch1 的表达水平从Ⅰ级胶质瘤逐渐升高至Ⅳ级胶质瘤。 Notch1 阳性患者的总生存率明显低于 Notch1 阴性患者。多因素分析表明 Notch1 是脑胶质瘤患者的独立预后因素。
这些研究结果证实了 Notch1 在脑胶质瘤中表达上调，并与肿瘤的进展有关。 Notch1 也可能成为一种独立于 WHO 分级的新的预后标志物。
