Xiamen University, College of Materials, Department of Biomaterials, Xiamen, People's Republic of China.
IET Nanobiotechnol. 2011 Mar;5(1):20-4. doi: 10.1049/iet-nbt.2010.0009.
The interaction of gold nanoparticles with Pfu DNA polymerase has been investigated by a number of biological, optical and electronic spectroscopic techniques. Polymerase chain reaction was performed to show gold nanoparticles' biological effect. Ultraviolet-visible and circular dichroism spectra analysis were applied to character the structure of Pfu DNA polymerase after conjugation with gold nanoparticles. X-ray photoelectron spectroscopy was used to investigate the bond properties of the polymerase-gold nanoparticles complex. The authors demonstrate that gold nanoparticles do not affect the amplification efficiency of polymerase chain reaction using Pfu DNA polymerase, and Pfu DNA polymerase displays no significant changes of the secondary structure upon interaction with gold nanoparticles. The adsorption of Pfu DNA polymerase to gold nanoparticles is mainly through Au-NH(2) bond and electrostatic interaction. These findings may have important implications regarding the safety issue as gold nanoparticles are widely used in biomedical applications.
已采用多种生物、光学和电子光谱技术研究了金纳米粒子与 Pfu DNA 聚合酶的相互作用。通过聚合酶链反应展示了金纳米粒子的生物学效应。应用紫外-可见和圆二色光谱分析对金纳米粒子偶联后 Pfu DNA 聚合酶的结构进行了表征。采用 X 射线光电子能谱研究了聚合酶-金纳米粒子复合物的键合性质。作者证明，金纳米粒子不会影响使用 Pfu DNA 聚合酶进行聚合酶链反应的扩增效率，并且 Pfu DNA 聚合酶在与金纳米粒子相互作用时其二级结构没有明显变化。Pfu DNA 聚合酶吸附到金纳米粒子上主要是通过 Au-NH(2)键和静电相互作用。这些发现对于金纳米粒子在生物医学应用中广泛使用的安全性问题具有重要意义。
