Liu Chong-Bin, Feng Yan-Hong, Ye Guang-Hua, Xiao Min
Institute of Nutrition and Health Ecology, School of Basic Medicine, Wenzhou Medical College, Wenzhou 325035, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Dec;28(12):891-4.
To explore arsenic-induced oxidative stress and the protective efficacy of α-lipoic acid and vitamin c.
50 male SD rats were randomly divided into 5 groups. Ten rats (the control group) were exposed to deionized water for 6 weeks, and the others were alone exposed to sodium arsenite (50 mg/L water) for 6 weeks, at the same time, three group rats were administered intragastrically (i.g.) with α-lipoic acid 10 mg×kg(-1)×d(-1) and vitamin C 25 mg×kg(-1)×d(-1) either alone or in combination. At the end of experiment, blood was drawn from abdominal aorta, and then the blood, brain and liver of rats were used for biochemical assays, including blood glutathione (GSH), δ-aminolevulinic acid dehydratase (δ-ALAD ), reactive oxygen species (ROS) and oxidized glutathione (GSSG) level. At the same time, the super oxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, catalase (CAT) activity, ATPase activity of brain and liver were determined. The caspase activity of brain were also determined.
There were a significant increase in ROS level (P < 0.05), but a significant decrease in δ-ALAD activity (P < 0.01) in the chronic arsenic toxicity model group compared with the control group. These alterations were marginally restored by co-administration of vitamin C and α-lipoic acid individually, while significant recovery was observed in the animals supplemented with both the antioxidants together with arsenite in rat (P < 0.05). At the same time, there was a significant increase in the ROS and TBARS level of the brain and liver (P < 0.05), and caspase activity of the brain (P < 0.05), while there was a significant decrease in antioxidant enzymes and ATPase activity on arsenite exposure in rats (P < 0.05). These alterations were also marginally restored by co-administration of vitamin C and α-lipoic acid individually, while significant recovery was observed in the animals supplemented with both the antioxidants together with arsenite in rat (P < 0.05).
Arsenite-induced oxidative stress can be significantly protected by co-administration of α-lipoic acid and vitamin C individually, but the best effects could be observed with combined administration of two antioxidants during arsenite exposure in animals. The dietary intervention of or supplementation with natural dietary nutrients is possible to prevent the effects of arsenic in populations of risk.
探讨砷诱导的氧化应激以及α-硫辛酸和维生素C的保护作用。
将50只雄性SD大鼠随机分为5组。10只大鼠（对照组）饮用去离子水6周，其余大鼠单独饮用含亚砷酸钠（50 mg/L水）的水6周，同时，三组大鼠分别单独或联合灌胃给予α-硫辛酸10 mg×kg⁻¹×d⁻¹和维生素C 25 mg×kg⁻¹×d⁻¹。实验结束时，从腹主动脉取血，然后将大鼠的血液、脑和肝脏用于生化检测，包括血液谷胱甘肽（GSH）、δ-氨基乙酰丙酸脱水酶（δ-ALAD）、活性氧（ROS）和氧化型谷胱甘肽（GSSG）水平。同时，测定脑和肝脏的超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GSH-Px）活性、过氧化氢酶（CAT）活性、ATP酶活性。还测定了脑的半胱天冬酶活性。
与对照组相比，慢性砷中毒模型组的ROS水平显著升高（P < 0.05），而δ-ALAD活性显著降低（P < 0.01）。单独给予维生素C和α-硫辛酸可使这些改变略有恢复，而在大鼠中同时补充两种抗氧化剂和亚砷酸钠时观察到显著恢复（P < 0.05）。同时，大鼠脑和肝脏的ROS和丙二醛水平显著升高（P < 0.05），脑的半胱天冬酶活性显著升高（P < 0.05），而暴露于亚砷酸钠时大鼠的抗氧化酶和ATP酶活性显著降低（P < 0.05）。单独给予维生素C和α-硫辛酸也可使这些改变略有恢复，而在大鼠中同时补充两种抗氧化剂和亚砷酸钠时观察到显著恢复（P < 0.05）。
单独给予α-硫辛酸和维生素C均可显著减轻亚砷酸盐诱导的氧化应激，但在动物亚砷酸盐暴露期间联合给予两种抗氧化剂效果最佳。通过饮食干预或补充天然膳食营养素可能预防砷对高危人群的影响。
