Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Program, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Arch Oral Biol. 2011 Jul;56(7):643-9. doi: 10.1016/j.archoralbio.2010.12.012. Epub 2011 Jan 15.
This study aimed to evaluate the influence of NaF (2, 10, 50 and 125 ppm F(-)) on the virulence factors and composition of Streptococcus mutans biofilms.
S. mutans UA159 biofilms were formed on saliva-coated hydroxyapatite discs. To assess the influence of NaF on the virulence factors of S. mutans biofilm cells, glycolytic pH drop, proton-permeability and F-ATPase activity assay were performed using 74 h old S. mutans biofilms. Glucosyltransferase (GTF) activity assay in suspension was also performed. To examine the influence of NaF on S. mutans biofilm composition, the biofilms were treated twice daily (5 min exposure/treatment) a total of five times during biofilm formation. After a total of 5 treatments, the biomass, colony forming unit (CFU) and polysaccharide composition of the treated 74h old S. mutans biofilms were analysed by microbiological and biochemical methods, and scanning electron microscopy.
NaF showed inhibitory effects on the acid production and acid tolerance of S. mutans biofilm cells at 10, 50 and 125 ppm F(-), compared to the vehicle control (P<0.05) and the treatments at these concentrations also affected the biomass, water-insoluble extracellular polysaccharides and intracellular iodophilic polysaccharides of the biofilms, compared to the vehicle control (P<0.05).
These results indicate that NaF (10, 50 and 125 ppm F(-)) has inhibitory effects on the virulence factors and composition of S. mutans biofilms, suggesting the potential use of these concentrations as an effective measure for controlling dental biofilms.
本研究旨在评估 NaF（2、10、50 和 125ppmF(-)）对变形链球菌生物膜毒力因子和组成的影响。
在唾液包被的羟磷灰石片上形成变形链球菌 UA159 生物膜。为了评估 NaF 对变形链球菌生物膜细胞毒力因子的影响，使用 74 小时龄的变形链球菌生物膜进行糖酵解 pH 下降、质子通透性和 F-ATP 酶活性测定。悬浮液中的葡糖基转移酶（GTF）活性测定也同时进行。为了研究 NaF 对变形链球菌生物膜组成的影响，在生物膜形成过程中，每天两次（5 分钟暴露/处理）共处理 5 次。在总共 5 次处理后，通过微生物学和生物化学方法以及扫描电子显微镜分析处理的 74 小时龄变形链球菌生物膜的生物量、菌落形成单位（CFU）和多糖组成。
与载体对照相比，10、50 和 125ppmF(-)浓度的 NaF 对变形链球菌生物膜细胞的产酸和耐酸能力均表现出抑制作用（P<0.05），且这些浓度的处理还影响了生物膜的生物量、水不溶性胞外多糖和细胞内亲碘多糖，与载体对照相比（P<0.05）。
这些结果表明，NaF（10、50 和 125ppmF(-)）对变形链球菌生物膜的毒力因子和组成具有抑制作用，表明这些浓度可用作控制牙生物膜的有效措施。
