Zen Igor, Delbem Alberto Carlos Botazzo, Hosida Thayse Yumi, Sampaio Caio, de Morais Leonardo Antônio, Martins Tamires Passadori, Monteiro Douglas Roberto, Pessan Juliano Pelim
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, Brazil.
Postgraduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente 19050-920, SP, Brazil.
Antibiotics (Basel). 2023 Aug 18;12(8):1333. doi: 10.3390/antibiotics12081333.
This study evaluated the antimicrobial effect of toothpastes containing 200 ppm fluoride (200F), xylitol (X, 16%), erythritol (E, 4%), and sodium trimetaphosphate (TMP, 0.25%), alone or in different associations, against (SM), (LC), (AI), and (CA). Suspensions of the micro-organisms were added to a BHI Agar medium. Five wells were made on each plate to receive toothpaste suspensions at different dilutions. Toothpastes containing no actives (placebo) or 1100 ppm F (1100F) were used as negative and positive controls. Two-way ANOVA and Tukey's HDS test were used ( < 0.05). For SM, the largest halo was for 200F+TMP at all dilutions, followed by the 200F+X+E toothpaste ( < 0.001). For LC, the overall trend showed that the polyols effectively inhibited microbial growth, and the association with the other compounds enhanced such effects ( < 0.001). For AI, a less-defined trend was observed. For CA, the experimental toothpaste (200F+X+E+TMP) was consistently more effective than the other treatments, followed by 200F+X+E ( < 0.001). The association of polyols and TMP in a low-fluoride toothpaste effectively reduced the growth of cariogenic micro-organisms (SM, CA, and LC), suggesting that this formulation could be an interesting alternative for children due to its low fluoride content.
本研究评估了含200 ppm氟化物（200F）、木糖醇（X，16%）、赤藓糖醇（E，4%）和三聚偏磷酸钠（TMP，0.25%）的牙膏单独或不同组合对变形链球菌（SM）、嗜酸乳杆菌（LC）、远缘链球菌（AI）和变异链球菌（CA）的抗菌效果。将微生物悬液添加到脑心浸液琼脂培养基中。在每个平板上制作五个孔，以接受不同稀释度的牙膏悬液。不含活性成分的牙膏（安慰剂）或含1100 ppm F（1100F）的牙膏用作阴性和阳性对照。使用双向方差分析和Tukey's HDS检验（P<0.05）。对于SM，在所有稀释度下，最大抑菌圈是200F+TMP牙膏，其次是200F+X+E牙膏（P<0.001）。对于LC，总体趋势表明多元醇有效地抑制了微生物生长，并且与其他化合物的组合增强了这种效果（P<0.001）。对于AI，观察到的趋势不太明确。对于CA，实验牙膏（200F+X+E+TMP）始终比其他处理更有效，其次是200F+X+E（P<0.001）。低氟牙膏中多元醇和TMP的组合有效地减少了致龋微生物（SM、CA和LC）的生长，表明由于其低氟含量，这种配方可能是儿童的一个有趣选择。
