美洲水貂（鼬属水貂）免疫球蛋白λ链cDNA的克隆与测序

[Cloning and sequencing of immunoglobin lambda-chain cDNA in american mink (Mustela vison].

作者信息

Naiakshin A M, Belousov E S, Taranin A V, Bogachev S S, Rogozin I B, Baranov O K

出版信息

Genetika. 1990 Aug;26(8):1527-31.

Abstract

cDNA library in the lambda gt11 phage was constructed using poly (A+)-mRNA from mink spleen as a template. Immunoscreening of the library allowed the identification of 2 lambda-related clones containing 370 and 803 bp insertion (lambda IGL-1 and lambda IGL-2). Analysis of the primary structure of lambda IGL-2 demonstrated that it contains a large portion of V lambda-segment, J lambda-segment, C lambda-gene and its 3'-untranslated part. The nucleotide sequences known for the immunoglobulin genes were compared to the sequence of the lambda IGL-2 clone. The highest degree of homology was established for the rabbit lambda-genes, this being 63, 94 and 72% for the RF3 region of V lambda-segment, J lambda-segment and C lambda-region, respectively.

摘要

以水貂脾脏的聚腺苷酸加尾（poly(A+)）信使核糖核酸（mRNA）为模板，构建了λgt11噬菌体中的互补脱氧核糖核酸（cDNA）文库。对该文库进行免疫筛选，鉴定出2个与λ相关的克隆，其插入片段分别为370和803碱基对（λIGL-1和λIGL-2）。对λIGL-2一级结构的分析表明，它包含大部分可变λ片段（Vλ-segment）、连接λ片段（Jλ-segment）、恒定λ基因（Cλ-gene）及其3'非翻译区。将已知的免疫球蛋白基因核苷酸序列与λIGL-2克隆的序列进行比较。与兔λ基因建立了最高程度的同源性，可变λ片段的RF3区域、连接λ片段和恒定λ区域分别为63%、94%和72%。