Khlebodarova T M, Matveeva N M, Serov O L, Najakshin A M, Belousov E S, Bogachev S V, Baranov O K
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk.
Mamm Genome. 1992;2(2):96-9. doi: 10.1007/BF00353856.
A cDNA library from mink spleen was constructed by use of the phage lambda gt11. The library was screened using polyvalent serum raised against the mink immunoglobulin lambda chain. As a result, several clones expressing mink immunoglobulin lambda light chains were identified. Sequencing of one of the clones with an 803 bp insert was performed. The insert comprised nearly the entire coding region for the mature lambda light immunoglobulin gene with the exception of the leader polypeptide and several amino acids of the FR1 region of the V segment. Compared with the rabbit, mouse and human lambda light immunoglobulin genes, the homology of the cloned sequence was found to be highest relative to the rabbit gene. With the cloned mink cDNA containing the C-region only as a probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of this mink cDNA sequence and mink chromosomes in the mink-Chinese hamster clone panel allowed us to assign the gene for the lambda light immunoglobulin constant polypeptide (IGLC) to mink Chromosome (Chr) 4.
利用λ噬菌体gt11构建了水貂脾脏的cDNA文库。使用针对水貂免疫球蛋白λ链产生的多价血清对该文库进行筛选。结果，鉴定出了几个表达水貂免疫球蛋白λ轻链的克隆。对其中一个插入片段为803 bp的克隆进行了测序。该插入片段几乎包含成熟λ轻免疫球蛋白基因的整个编码区，但不包括前导多肽和V区段FR1区域的几个氨基酸。与兔、小鼠和人类的λ轻免疫球蛋白基因相比，发现克隆序列与兔基因的同源性最高。以仅含C区的克隆水貂cDNA为探针，研究了水貂-中国仓鼠杂交克隆的DNA。对该水貂cDNA序列和水貂-中国仓鼠克隆组中的水貂染色体进行分离分析的结果，使我们能够将λ轻免疫球蛋白恒定多肽（IGLC）基因定位到水貂4号染色体（Chr）上。
