Marine Microbiology Group, Department of Ecology and Marine Resources, Institut Mediterrani d'Estudis Avançats (CSIC-UIB), E-07190 Esporles, Mallorca, Illes Balears, Spain.
Syst Appl Microbiol. 2011 Feb;34(1):69-75. doi: 10.1016/j.syapm.2010.11.012. Epub 2011 Jan 15.
The moderately halophilic, cultivable fraction of prokaryotes thriving in hypersaline sediments of a solar saltern in Mallorca, Spain, has been studied by means of different cultivation media. A set of 374 isolates retrieved with six different culture conditions was screened, using whole-cell MALDI-TOF MS analysis to classify them into 25 phenotypic clusters at 52% similarity. The phylogenetic inference, made from comparative sequence analyses of the 16S rRNA genes of selected strains, indicated that each phenotypic cluster was comprised of a genealogically homogeneous set of strains. DNA-DNA hybridization (DDH) results among selected strains confirmed that each MALDI-TOF cluster encompassed members of the same species. On the other hand, the intra-cluster diversity, measured by several RAPD (Random Amplified Polymorphic DNA) amplifications, indicated that the clusters corresponded to several populations of the same phylogenetic unit coexisting in the same environment. The results encourage the use of MALDI-TOF MS for further exhaustive studies of the cultivable diversity of hypersaline environments.
在西班牙马略卡岛盐田的高盐沉积物中，通过不同的培养介质研究了可培养的中度嗜盐原核生物的可培养部分。使用六种不同的培养条件获得的一组 374 个分离株进行了筛选，使用全细胞 MALDI-TOF MS 分析将它们分为 25 个表型群，相似度为 52%。从选定菌株的 16S rRNA 基因的比较序列分析进行的系统发育推断表明，每个表型群由一组系统发育上同质的菌株组成。选择的菌株之间的 DNA-DNA 杂交 (DDH) 结果证实，每个 MALDI-TOF 群都包含相同物种的成员。另一方面，通过多个 RAPD（随机扩增多态性 DNA）扩增测量的聚类内多样性表明，这些聚类对应于同一进化单位的多个种群在相同环境中共存。这些结果鼓励使用 MALDI-TOF MS 对高盐环境的可培养多样性进行进一步的详尽研究。
