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基质辅助激光解吸电离飞行时间质谱在食源细菌鉴定中的应用

Application of MALDI-TOF MS for the Identification of Food Borne Bacteria.

作者信息

Pavlovic Melanie, Huber Ingrid, Konrad Regina, Busch Ulrich

机构信息

Bavarian Health and Food Safety Authority, 85354 Oberschleißheim, Germany.

出版信息

Open Microbiol J. 2013 Nov 15;7:135-41. doi: 10.2174/1874285801307010135. eCollection 2013.

DOI:10.2174/1874285801307010135
PMID:24358065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3866695/
Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful tool for the routine identification of clinical isolates. MALDI-TOF MS based identification of bacteria has been shown to be more rapid, accurate and cost-efficient than conventional phenotypic techniques or molecular methods. Rapid and reliable identification of food-associated bacteria is also of crucial importance for food processing and product quality. This review is concerned with the applicability of MALDI-TOF MS for routine identification of foodborne bacteria taking the specific requirements of food microbiological laboratories and the food industry into account. The current state of knowledge including recent findings and new approaches are discussed.

摘要

基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）最近已成为临床分离株常规鉴定的有力工具。基于MALDI-TOF MS的细菌鉴定已被证明比传统的表型技术或分子方法更快速、准确且具有成本效益。快速可靠地鉴定与食品相关的细菌对于食品加工和产品质量也至关重要。本综述考虑到食品微生物实验室和食品行业的特定要求，探讨了MALDI-TOF MS在食源细菌常规鉴定中的适用性。讨论了当前的知识状态，包括最近的发现和新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/8a4b071cb43e/TOMICROJ-7-135_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/d6f82e3a870d/TOMICROJ-7-135_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/8cb1fa36199f/TOMICROJ-7-135_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/5cd22d5f43ba/TOMICROJ-7-135_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/8a4b071cb43e/TOMICROJ-7-135_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/d6f82e3a870d/TOMICROJ-7-135_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/8cb1fa36199f/TOMICROJ-7-135_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/5cd22d5f43ba/TOMICROJ-7-135_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8da5/3866695/8a4b071cb43e/TOMICROJ-7-135_F4.jpg

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