对来自阿劳卡河沉积物（哥伦比亚）的放线菌群落的表征揭示了低丰度分离物中存在的抗菌潜力。
Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates.
作者信息
Arango Carolina, Acosta-Gonzalez Alejandro, Parra-Giraldo Claudia M, Sánchez-Quitian Zilpa A, Kerr Russell, Díaz Luis E
机构信息
Universidad de La Sabana, Campus del Puente del Común, Km 7 Autopista Norte de Bogotá, Chia, Colombia.
University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.
出版信息
Open Microbiol J. 2018 May 31;12:181-194. doi: 10.2174/1874285801812010181. eCollection 2018.
INTRODUCTION
New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.
MATERIAL AND METHODS
An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.
RESULTS
Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant , Vancomycin-resistant , extended-spectrum β-lactamase , and clinical isolates of and Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.
CONCLUSION
The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.
引言
已经出现了新的策略来进行快速有效的筛选，以选择具有生物活性潜力的微生物。本研究表明，物理化学预处理与通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对微生物库进行分类学重复排除相结合，有助于检测具有作为抗菌剂来源潜力的低丰度放线菌。
材料与方法
描述了来自哥伦比亚奥里诺科河热带河底沉积物的一个未研究的微生物群落，采用了一种扩展培养策略，包括物理化学预处理、生物筛选以及通过MALDI-TOF MS方法进行分类学重复排除。
结果
培养出了类似放线菌的分离株（790株），并评估了它们对耐甲氧西林、耐万古霉素、超广谱β-内酰胺酶以及临床分离的金黄色葡萄球菌和大肠杆菌的抗菌活性。根据16S rDNA分析，发现78株属于链霉菌科的分离株具有抗菌活性，并且通过MALDI-TOF MS被归类为低丰度放线菌。
结论
结果表明，物理化学预处理与通过MALDI-TOF MS对微生物库进行分类学重复排除相结合，有助于检测具有作为抗菌剂来源潜力的低丰度放线菌。
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