氨基酸及其水溶液的拉曼光谱。

Raman spectra of amino acids and their aqueous solutions.

机构信息

Department of Chemical Engineering, Shanghai University, Shanghai, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2011 Mar;78(3):1187-95. doi: 10.1016/j.saa.2010.12.079. Epub 2010 Dec 30.

DOI:10.1016/j.saa.2010.12.079

Abstract

Amino acids are the basic "building blocks" that combine to form proteins and play an important physiological role in all life-forms. Amino acids can be used as models for the examination of the importance of intermolecular bonding in life processes. Raman spectra serve to obtain information regarding molecular conformation, giving valuable insights into the topology of more complex molecules (peptides and proteins). In this paper, amino acids and their aqueous solution have been studied by Raman spectroscopy. Comparisons of certain values for these frequencies in amino acids and their aqueous solutions are given. Spectra of solids when compared to those of the solute in solution are invariably much more complex and almost always sharper. We present a collection of Raman spectra of 18 kinds of amino acids (L-alanine, L-arginine, L-aspartic acid, cystine, L-glutamic acid, L-glycine, L-histidine, L-isoluecine, L-leucine, L-lysine, L-phenylalanine, L-methionone, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, L-valine) and their aqueous solutions that can serve as references for the interpretation of Raman spectra of proteins and biological materials.

摘要

氨基酸是构成蛋白质的基本“积木”，在所有生命形式中都发挥着重要的生理作用。氨基酸可以作为模型，用于研究分子间键合在生命过程中的重要性。拉曼光谱可用于获取有关分子构象的信息，为更复杂分子（肽和蛋白质）的拓扑结构提供有价值的见解。本文通过拉曼光谱研究了氨基酸及其水溶液。给出了这些频率在氨基酸及其水溶液中的某些值的比较。与溶液中溶质的光谱相比，固体的光谱总是复杂得多，而且几乎总是更尖锐。我们提供了 18 种氨基酸（L-丙氨酸、L-精氨酸、L-天冬氨酸、半胱氨酸、L-谷氨酸、L-甘氨酸、L-组氨酸、L-异亮氨酸、L-亮氨酸、L-赖氨酸、L-苯丙氨酸、L-甲硫氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸、L-缬氨酸）及其水溶液的拉曼光谱图谱，可作为蛋白质和生物材料拉曼光谱解释的参考。