Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea.
Blood Purif. 2011;31(4):281-8. doi: 10.1159/000321889. Epub 2011 Jan 14.
An increased percentage of pro-inflammatory CD14(+)CD16(+) monocytes might contribute to inflammation in hemodialysis (HD) patients. The purpose of the study was to evaluate the possible contribution of pro-inflammatory monocytes to inflammation in HD patients and also to evaluate the effect of on-line hemodiafiltration (HDF).
Flow cytometric detection of monocytes in patients undergoing HD, on-line HDF and healthy controls as well as plasma cytokines and cytokine mRNA measurement were performed.
Percent pro-inflammatory monocytes, plasma cytokines and cytokine mRNA significantly increased in HD patients. Intracellular cytokine staining showed pro-inflammatory monocytes were the predominant source of tumor necrosis factor-α. Percent pro-inflammatory monocytes positively correlated with plasma inflammatory cytokines. Percent pro-inflammatory monocytes, plasma cytokines and cytokine mRNA significantly decreased in on-line HDF patients.
Increased pro-inflammatory monocytes are likely to contribute to inflammation in HD patients, and beneficial effect of on-line HDF might be partially mediated by modulating the inflammatory response.
促炎型 CD14(+)CD16(+)单核细胞比例的增加可能导致血液透析（HD）患者的炎症反应。本研究旨在评估促炎型单核细胞对 HD 患者炎症反应的可能贡献，同时评估在线血液透析滤过（HDF）的作用。
采用流式细胞术检测 HD 患者、在线 HDF 患者和健康对照者的单核细胞，检测血浆细胞因子和细胞因子 mRNA。
HD 患者的促炎型单核细胞比例、血浆细胞因子和细胞因子 mRNA 均显著升高。细胞内细胞因子染色显示促炎型单核细胞是肿瘤坏死因子-α的主要来源。促炎型单核细胞比例与血浆炎性细胞因子呈正相关。在线 HDF 患者的促炎型单核细胞比例、血浆细胞因子和细胞因子 mRNA 均显著降低。
促炎型单核细胞可能导致 HD 患者的炎症反应，在线 HDF 的有益作用可能部分通过调节炎症反应来介导。
