Stettin A, Schultze J L, Stechemesser E, Berg P A
Medizinischen Klinik, Abteilung II, Universität Tübingen.
Klin Wochenschr. 1990 Sep 14;68(18):896-900. doi: 10.1007/BF01649034.
The humoral response components of an aqueous mistletoe extract (HM) was evaluated in 23 tumor patients who had been treated from 2 months up to 6 years with increasing dosages of HM. IgG antibodies against mistletoe lectin and other components of this extract were detected by ELISA, immunodiffusion, and blotting technique, using either the aqueous extract (HM) or a purified lectin preparation (ML). Their activity depended upon dosage of HM and length of therapy. No anti-HM/ML antibodies of the IgM type could be detected. Immunoblotting revealed lectin-specific antigens at 62 kD, 33k D, and 29 kD. In the presence of ML or HM, PHA-induced proliferation of normal lymphocytes was decreased in a dose-dependent manner; this effect was neutralized by adding the IgG fraction from pooled anti-HM-antibody-positive sera, indicating that the cytotoxic effect of lectins was eliminated by these specific antibodies. In view of these findings, it is questionable whether exposing tumor cells to mistletoe extracts in vivo exerts the same direct effect on tumor cells that is observed in vitro.
对23名肿瘤患者的体液免疫反应成分进行了评估，这些患者接受了2个月至6年不等剂量递增的槲寄生水提取物（HM）治疗。采用酶联免疫吸附测定（ELISA）、免疫扩散和印迹技术，使用水提取物（HM）或纯化的凝集素制剂（ML），检测针对槲寄生凝集素和该提取物其他成分的IgG抗体。其活性取决于HM的剂量和治疗时间。未检测到IgM型抗HM/ML抗体。免疫印迹显示在62kD、33kD和29kD处有凝集素特异性抗原。在存在ML或HM的情况下，PHA诱导的正常淋巴细胞增殖呈剂量依赖性降低；通过添加来自混合抗HM抗体阳性血清的IgG组分可中和这种效应，表明这些特异性抗体消除了凝集素的细胞毒性作用。鉴于这些发现，肿瘤细胞在体内暴露于槲寄生提取物是否会对肿瘤细胞产生与体外观察到的相同直接作用值得怀疑。
