Saha Chaitrali, Das Mrinmoy, Stephen-Victor Emmanuel, Friboulet Alain, Bayry Jagadeesh, Kaveri Srini V
Institut National de la Santé et de la Recherche Médicale Unité 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de Médicine, Paris F-75006, France.
Université de Technologie de Compiègne, UMR CNRS 6022, Compiègne F-60205, France.
Molecules. 2016 Jul 14;21(7):912. doi: 10.3390/molecules21070912.
Extracts of Viscum album (VA); a semi-parasitic plant, are frequently used in the complementary therapy of cancer and other immunological disorders. Various reports show that VA modulates immune system and exerts immune-adjuvant activities that might influence tumor regression. Currently, several therapeutic preparations of VA are available and hence an insight into the mechanisms of action of different VA preparations is necessary. In the present study, we performed a comparative study of five different preparations of VA on maturation and activation of human dendritic cells (DCs) and ensuing CD4⁺ T cell responses. Monocyte-derived human DCs were treated with VA Qu Spez, VA Qu Frf, VA M Spez, VA P and VA A. Among the five VA preparations tested VA Qu Spez, a fermented extract with a high level of lectins, significantly induced DC maturation markers CD83, CD40, HLA-DR and CD86, and secretion of pro-inflammatory cytokines such as IL-6, IL-8, IL-12 and TNF-α. Furthermore, analysis of T cell cytokines in DC-T cell co-culture revealed that VA Qu Spez significantly stimulated IFN-γ secretion without modulating regulatory T cells and other CD4⁺ T cytokines IL-4, IL-13 and IL-17A. Our study thus delineates differential effects of VA preparations on DC maturation; function and T cell responses.
槲寄生提取物（VA）；一种半寄生植物，常用于癌症和其他免疫紊乱的辅助治疗。各种报告表明，VA可调节免疫系统并发挥免疫佐剂活性，这可能影响肿瘤消退。目前，有几种VA治疗制剂可供使用，因此有必要深入了解不同VA制剂的作用机制。在本研究中，我们对五种不同的VA制剂对人树突状细胞（DC）的成熟和激活以及随后的CD4⁺T细胞反应进行了比较研究。单核细胞衍生的人DC用VA Qu Spez、VA Qu Frf、VA M Spez、VA P和VA A处理。在所测试的五种VA制剂中，VA Qu Spez是一种含有高水平凝集素的发酵提取物，可显著诱导DC成熟标志物CD83、CD40、HLA-DR和CD86，并分泌促炎细胞因子如IL-6、IL-8、IL-12和TNF-α。此外，对DC-T细胞共培养中T细胞细胞因子的分析表明，VA Qu Spez可显著刺激IFN-γ分泌而不调节调节性T细胞和其他CD4⁺T细胞细胞因子IL-4、IL-13和IL-17A。因此，我们的研究描述了VA制剂对DC成熟、功能和T细胞反应的不同影响。
