Department of Agronomy, Purdue University, Lilly Hall, 915 West State Street, West Lafayette, IN 47907, USA.
Mol Biol Rep. 2011 Nov;38(8):5281-4. doi: 10.1007/s11033-011-0677-6. Epub 2011 Jan 18.
Extraction of plant genomic DNA for subsequent genetic studies is often lengthy and difficult. In addition, it requires the use of toxic reagents that remove secondary plant products, which otherwise interfere with the polymerase chain reaction steps. Here we describe a simple cost-efficient one-step protocol for PCR-based analysis in Arabidopsis thaliana. This protocol is quick, performed at room temperature without the need for DNA extraction. Potential applications in higher plants are here discussed.
为后续的遗传研究提取植物基因组 DNA 通常既冗长又困难。此外，它还需要使用有毒试剂来去除次级植物产物，否则这些产物会干扰聚合酶链反应步骤。在此，我们描述了一种用于拟南芥基于 PCR 的分析的简单、经济高效的一步法方案。该方案快速，在室温下进行，无需 DNA 提取。本文还讨论了该方案在高等植物中的潜在应用。
