Department of Mechanical Engineering, University College London, Torrington Place, London, WC 1E 7JE, UK.
J Mater Sci Mater Med. 2011 Mar;22(3):491-6. doi: 10.1007/s10856-010-4226-y. Epub 2011 Jan 18.
Nano-sized titanium containing hydroxyaptite has been prepared, the particle size of nanoTiHA was shown to be 12-20 nm in width and 30-40 nm in length, smaller than that of nanoHA. X-ray diffraction analysis revealed the phase purity of nanoTiHA produced. Antimicrobical assays demonstrated that nanoTiHA has excellent growth inhibitory properties, and is able to inhibit the growth of all bacterial strains tested, both Gram-negative and Gram-positive species, including multi-antibiotic resistant EMRSA 15 and EMRSA 16 'superbugs'. Biocidal activity against all four Staphylococcus spp was also shown at the concentration tested. Nanostuctured TiHA coating was successfully deposited onto Ti surfaces using EHDA spraying under optimized processing conditions with the thickness of the coating being further controlled by the spraying time. All of the nanoTiHA coated Ti surfaces were able to support human osteoblast (HOB) cell attachment and growth. The coating thickness did not significantly influence the proliferation of HOB cells on nanoTiHA coatings, while the ability of nanoTiHA coating to support HOB cell differentiation was demonstrated from the alkaline phosphatase activity. Our study showed that nanoTiHA has excellent anti-bacterial properties and the thin nanoTiHA coating was also able to support the attachment, growth and differentiation of HOB cells. Therefore, nanoTiHA coating could pave the way for the development of the next generation of dental and orthopedic implants by offering anti-infection potential in addition to osteoconductivity.
已制备出含纳米级钛的羟基磷灰石，纳米 TiHA 的粒径显示为 12-20nm 宽，30-40nm 长，小于纳米 HA。X 射线衍射分析显示了所制备的纳米 TiHA 的相纯度。抗菌测定表明纳米 TiHA 具有优异的生长抑制特性，能够抑制所有测试的细菌菌株的生长，包括革兰氏阴性和革兰氏阳性物种，包括多抗生素耐药性 EMRSA 15 和 EMRSA 16“超级细菌”。在测试浓度下，对所有四种金黄色葡萄球菌也显示出杀菌活性。在优化的处理条件下，通过 EHDA 喷涂成功地将纳米结构的 TiHA 涂层沉积到 Ti 表面上，通过喷涂时间进一步控制涂层的厚度。所有涂覆纳米 TiHA 的 Ti 表面都能够支持人成骨细胞（HOB）的附着和生长。涂层厚度对 HOB 细胞在纳米 TiHA 涂层上的增殖没有显著影响，而纳米 TiHA 涂层支持 HOB 细胞分化的能力则通过碱性磷酸酶活性得到证明。我们的研究表明，纳米 TiHA 具有优异的抗菌性能，并且薄的纳米 TiHA 涂层还能够支持 HOB 细胞的附着、生长和分化。因此，纳米 TiHA 涂层通过提供抗感染潜力以及骨传导性，可以为下一代牙科和骨科植入物的开发铺平道路。
