不同品种栀子中栀子苷和藏红花素-1的测定
[Determination of geniposide and croein-1 in different breed of fructus gardeniae].
作者信息
Luo Guang-Ming, Chen Yan, Li Xia, Yang Ya-Qin, Zeng Jin-Xiang, Wu Bo, Zhu Ji-Xiao, Zhu Yu-Ye
机构信息
Jiangxi University of Traditional Chinese Medicins, Nanchang 330004, China.
出版信息
Zhong Yao Cai. 2010 Sep;33(9):1376-8.
OBJECTIVE
To determinate geniposide and croein-1 in Fructus Gardeniae from different breeds.
METHODS
RP-HPLC method was adopted. The chromatographic separation was performed on an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 microm, Agilent). The mobile phase was a mixture of acetonitrile-0.1% phosphoric acid aqueous solution in gradient elution. The wavelength of 238 nm and 440 nm was selected to determinate geniposide and crocins simultaneously.
RESULT
The contents of geniposide and croein-1 in different breeds of Fructus Gardeniae are different.
CONCLUSION
This method is simple and repeatable, and could be used for the quality control of Fructus Gardeniae.
目的
测定不同品种栀子中栀子苷和西红花苷-1的含量。
方法
采用反相高效液相色谱法。色谱分离在Eclipse XDB-C18柱（250 mm×4.6 mm，5μm，安捷伦）上进行。流动相为乙腈-0.1%磷酸水溶液的混合液，梯度洗脱。选择238 nm和440 nm波长同时测定栀子苷和西红花苷。
结果
不同品种栀子中栀子苷和西红花苷-1的含量不同。
结论
该方法简便、重复性好，可用于栀子的质量控制。
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