[高效液相色谱-蒸发光散射法测定栀子不同炮制品中京尼平苷、西红花苷和西红花酸的含量]
[Determination of geniposide, crocin and crocetin in different processing products of fructus gardeniae by HPLC-ELSD].
作者信息
Chen Yan, Yang Zhong-Lin, Zhang Lei-Hong, Liu Su-Juan, Zhang Xian-Tao
机构信息
Key Laboratory of Modem Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
出版信息
Zhong Yao Cai. 2011 May;34(5):687-90.
OBJECTIVE
To develop a HPLC- ELSD method for determination the contents of geniposide, crocin and crocetin in different processing products of Fructus Gardeniae.
METHODS
The separation was performed in the HyperClone ODS C18 column (250 mm x 4. 6 mm, 5 microm) with linear gradient elution using methanol-water and 0.05% phosphoric acid in water, the flowing rate was 0.8 mL/min, the column temperature was 30 degrees C, and the ELSD parameter was as follow: 70 degrees C as atomization temperature and 2.0 L/min as the gas flowing rate.
RESULTS
The contents of geniposide and crocin in raw, yellowish, carbocoal and scorched Fructus Gardeniae decreased with the deepening of processing degree. However, the content of crocetin in carbocoal and scorched Fructus Gardeniae increased comparing with the raw one.
CONCLUSION
This is a simple and credible quality control method, and can be used for the quality control and comprehensive evaluation for different processed products of Fructus Gardeniae.
目的
建立高效液相色谱-蒸发光散射检测法测定不同炮制品栀子中栀子苷、西红花苷和西红花酸的含量。
方法
采用HyperClone ODS C18柱（250 mm×4.6 mm，5μm），以甲醇-水和0.05%磷酸水溶液进行线性梯度洗脱，流速为0.8 mL/min，柱温为30℃，蒸发光散射检测器参数如下：雾化温度70℃，气体流速2.0 L/min。
结果
生栀子、炒黄栀子、焦栀子和栀子炭中栀子苷和西红花苷的含量随炮制程度加深而降低。然而，焦栀子和栀子炭中西红花酸的含量与生栀子相比有所增加。
结论
该方法简便可靠，可用于栀子不同炮制品的质量控制和综合评价。
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