Liu Chun-Lan, Li Yang, Du Ning, Huang Xiao, Wang Wen-Shu
College of Life and Environmental Science, MINZU University of China, Beijing 100081, China.
Zhong Yao Cai. 2010 Sep;33(9):1414-6.
The study was to provided the reference for further development and utilization of Ligularia hodgsonii.
The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water, and prectated by ethanol. The crude polysaccharide has been fractionted and purified, so LW21 was got. Its homogeneity was confirmed by chromatography on Sepharose CL-4B, paper chromatography and cellulous actate electrophoresis. The scavenging effets of the polysacehardes on * OH and O2-* were determined by fluorescence method.
Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW.
The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities, and their antioxidant activities had certain dose-relationship.
本研究旨在为蹄叶橐吾的进一步开发利用提供参考。
采用热水提取法从蹄叶橐吾中提取粗多糖LW，并用乙醇沉淀。对粗多糖进行分级和纯化，得到LW21。通过Sepharose CL-4B柱色谱、纸色谱和纤维素醋酸酯电泳对其纯度进行鉴定。采用荧光法测定多糖对·OH和O2-·的清除作用。
抗氧化实验表明，LW和LW21对超氧阴离子自由基和羟基自由基均有清除作用，且LW21对超氧阴离子自由基和羟基自由基的清除能力高于LW。
蹄叶橐吾多糖具有较强的抗氧化活性，且其抗氧化活性与剂量有一定的关系。
