Liu Chun-lan, Liu Hai-qing, Deng Yi-hong, Liu Ming-liang
College of Life and Environmental Science, Central University for Nationalitites, Beijing 100081, China.
Zhong Yao Cai. 2006 Feb;29(2):151-4.
The crude polysaccharide was extracted from Leave in Hippohae rhamnoides L. with hot water, and precipitated by ethanol. The crude polysaccharide has been fractionated by acidic ethanol. Three fractions (SJ1, SJ2, SJ3) were got respectively. SJ2 deproteinizationed by the combined methods of enzyme and Seveage, purified by DEAE-Sephadex A-25 gel filtration. PC analysis indicated that SJ22 is composed of Xyl,Ara,Glc,Gal,GaL A. The identification of purify by Sepharose CL-4B, paper chromatography and cellulous acetate electrophoresis showed it was homogeneous. Typical absorption of polysaccharides was shown in its IR spectrum. It contained a-glucosidic bonds by IR analysis. It had typical absorption of protein by UV scaning. SJ22 is first isolated from Leave in Hippohae rhamnoides L Scavenging free radical experiment showed that SJ22 was effective in scavenging superoxideradical and hydroxylradical, but only a little effective in scavenging lipid radical.
以沙棘叶为原料，采用热水浸提法提取粗多糖，乙醇沉淀，再用酸性乙醇分级，分别得到3个级分（SJ1、SJ2、SJ3）。其中，SJ2采用酶法和 Sevage 法相结合进行脱蛋白，经 DEAE-Sephadex A-25 凝胶柱层析纯化。纸层析分析表明，SJ22 由木糖（Xyl）、阿拉伯糖（Ara）、葡萄糖（Glc）、半乳糖（Gal）、半乳糖醛酸（Gal A）组成。经 Sepharose CL-4B 柱层析、纸层析和醋酸纤维素薄膜电泳鉴定为均一组分。红外光谱显示出典型的多糖吸收峰，红外分析表明其含有α-糖苷键，紫外扫描呈现出典型的蛋白质吸收峰。SJ22 是首次从沙棘叶中分离得到的。清除自由基实验表明，SJ22 对超氧阴离子自由基和羟自由基具有较好的清除作用，但对脂质自由基的清除作用较弱。
