Wang Li, Zhao Ying
Heilongjiang University of Chinese Medicine, Harbin 150076, China.
Zhong Yao Cai. 2010 Sep;33(9):1442-4.
To investigate the effect of decoction method on pharmacokinetics of Puerarin by determining Puerarin concentration of serum after administration of Gegenqinlian in single (D) and mixed decoction (H) to dogs.
HPLC method was established to determine serum concentration of Puerarin and pharmacokinetic parameters were calculated.
The parameters in dogs after administration of H and D were as follows: C(max) was (5.164 +/- 0.637) and (5.383 +/- 0.818) microg/mL, t(max) was (1.10 +/- 0.374) and (1.25 +/- 0.274) h, AUC(0-t) was (25.76 +/- 5.48) and (27.97 +/- 7.16) microg/(h x mL), respectively.
Decoction method has significant effect on pharmacokinetic parameters of puerarin in dogs.
通过测定葛根芩连汤单煎（D）与合煎（H）后犬血清中葛根素的浓度，研究煎法对葛根素药代动力学的影响。
建立高效液相色谱法测定血清中葛根素浓度，并计算药代动力学参数。
H组与D组给药后犬的药代动力学参数如下：C(max)分别为(5.164±0.637)和(5.383±0.818)μg/mL，t(max)分别为(1.10±0.374)和(1.25±0.274)h，AUC(0-t)分别为(25.76±5.48)和(27.97±7.16)μg/(h·mL)。
煎法对犬体内葛根素的药代动力学参数有显著影响。
