Xing Dian-Xia, Liu Xian-Ling, Xue Cun-Kuan, Huang Qi, Liu Zhi-Gao, Xiong Liang
Chongqing Three Gorges Central Hospital, Chongqing 404000, China.
Zhong Yao Cai. 2010 Sep;33(9):1445-9.
To observe the estrogenic effect of formononetin and its effect on the expressions of atrial estrogen receptor subtypes alpha and beta (ERalpha and ERbeta).
50 femal rats were randomly divided into five groups: sham group, model group, nilestriol group, formononetin groups of low and high dose. Rats in sham group were cut a piece of fat before closing the abdomen, the others were ovariectomized. Vaginal exfoliated cell were observed from the fifth day to the tenth after operation to test if the model is successful. The sham and model group were given nomal saline in 10 mL/kg by gavage, the remaining three groups were given nilestriol 2.5 mg/(kg x w), low [20 mg/(kg x d) land high dose [100 mg/(kg x d)) of formononetin by gavage respectively. In the 8th week, vaginal exfoliated cell were observed, then decapitated the rats, removed the uterus, weighed and take wright staining microscopy. The relative expressions of ERalpha and ERbeta of right atrium were detected by RT-PCR.
The vaginal cells exhibit a change of estrus after had been fed with high dose of formononetin after 8 weeks. Formononetin increase the uterus coefficient and the expression of atrial ERbeta (P < 0.01), but it dose not have any effect on the expression of ERalpha (P > 0.05).
Formononetin have estrogenic effect in ovariectomized rats, and it can markedly upregulate the expression of rats' atrial ERbeta.
观察刺芒柄花素的雌激素样作用及其对心房雌激素受体α和β（ERα和ERβ）表达的影响。
将50只雌性大鼠随机分为五组：假手术组、模型组、尼尔雌醇组、刺芒柄花素低剂量组和高剂量组。假手术组大鼠在关腹前切除一块脂肪，其余大鼠行卵巢切除术。术后第5天至第10天观察阴道脱落细胞，以检测模型是否成功。假手术组和模型组大鼠以10 mL/kg的生理盐水灌胃，其余三组分别以尼尔雌醇2.5 mg/(kg·w)、刺芒柄花素低剂量[20 mg/(kg·d)]和高剂量[100 mg/(kg·d)]灌胃。第8周时，观察阴道脱落细胞，然后断头处死大鼠，取出子宫，称重并进行瑞氏染色显微镜检查。通过RT-PCR检测右心房ERα和ERβ的相对表达。
高剂量刺芒柄花素灌胃8周后，阴道细胞出现动情周期变化。刺芒柄花素增加子宫系数和心房ERβ的表达（P<0.01），但对ERα的表达无影响（P>0.05）。
刺芒柄花素对去卵巢大鼠有雌激素样作用，且能显著上调大鼠心房ERβ的表达。
