Liu Xiang-Hong, Hou Da-Bin, Zhao Na, Wang Bo
Life Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010, China.
Zhong Yao Cai. 2010 Sep;33(9):1484-7.
In this study, microwave-assisted extraction techniques of flavonoids fom Aralia cordata were studied by the single factor analysis and orthogonal test, then the property antioxidant activities of flavonoids extracts were evaluated by two antioxidant assays, for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging and reducing power. The results showed that the optimum conditions were as follows: twice extracting Aralia cordata with 75 times volume (V/W) of 30% ethanol at 140W of microwave for 60 min at 80 degrees C. Under these conditions, the extraction yield was 1.35%. The flavonoids extracts could significantly scavenge DPPH and superoxide anion free radicals in dose-dependent manner, meanwhile a linear correlation between its concentration and reducing power was also observed (R2 = 0.9855).
本研究采用单因素分析和正交试验对龙牙楤木黄酮类化合物的微波辅助提取工艺进行了研究，然后通过1,1-二苯基-2-苦基肼（DPPH）自由基清除和还原能力两种抗氧化测定方法对黄酮类提取物的抗氧化活性进行了评价。结果表明，最佳条件如下：在80℃下，以140W微波功率，用75倍体积（V/W）的30%乙醇对龙牙楤木进行两次提取，每次60分钟。在此条件下，提取率为1.35%。黄酮类提取物能以剂量依赖的方式显著清除DPPH和超氧阴离子自由基，同时其浓度与还原能力之间也存在线性关系（R2 = 0.9855）。
