College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Molecules. 2012 Sep 21;17(9):11281-91. doi: 10.3390/molecules170911281.
In the present study, antioxidant properties of flavonoids and polysaccharides from tobacco (Nicotiana tabacum L.) leaves were evaluated in several in vitro systems, e.g., scavenging activities on hydroxyl, superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and reducing power. Flavonoids showed much better activity than polysaccharides in scavenging activities on free radicals. When compared to the positive control, ascorbic acid, both showed weaker antioxidant potential. However, flavonoids possessed comparable superoxide anion, DPPH and ABTS radical scavenging abilities to ascorbic acid at high concentration (600 μg/mL). Meanwhile, it was found that flavonoids had prominent effects on the reducing power, which was equivalent to ascorbic acid, and was significantly higher than polysaccharides. These results clearly indicate that flavonoids are effective in scavenging free radicals and have the potential to be powerful antioxidants. Thus, tobacco leaves could be considered as a potential source of natural antioxidants for food, pharmaceutical, cosmetics or nutraceutical industries.
在本研究中，评估了来自烟草（Nicotiana tabacum L.）叶子的类黄酮和多糖的抗氧化特性，在几种体外系统中，例如，清除羟自由基、超氧阴离子自由基、1,1-二苯基-2-苦基肼（DPPH）自由基和 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基的活性，以及还原能力。类黄酮在清除自由基的活性方面比多糖表现出更好的活性。与阳性对照物抗坏血酸相比，两者的抗氧化潜力都较弱。然而，在高浓度（600μg/mL）下，类黄酮具有与抗坏血酸相当的超氧阴离子、DPPH 和 ABTS 自由基清除能力。同时，发现类黄酮对还原能力有显著影响，与抗坏血酸相当，且明显高于多糖。这些结果清楚地表明，类黄酮在清除自由基方面有效，并且具有成为强大抗氧化剂的潜力。因此，烟草叶可以被认为是食品、制药、化妆品或营养保健品行业天然抗氧化剂的潜在来源。
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