[大孔吸附树脂分离纯化莲子心中生物碱的工艺研究]
[Separation and purification processes of alkaloids from Lotus plumule with macroporous adsorption resin].
作者信息
Liu Shao, Lei Peng, Li Xin-Zhong, Chen Ying-Hua
机构信息
Department of Pharmacy of Xiangya Hospital, Center South University, Hunan 410008, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 May;32(10):912-5.
OBJECTIVE
To explore the optimal separation of the alkaloids from Lotus plumule by selecting appropriate macroporous adsorption resins.
METHOD
To evaluated the separating efficiency by measuring the adsorption ratio, eluting ratio and the concentration of neferine.
RESULT
The LSA -5B macroporous adsorption resin prolided the optimal separating efficiency. The best adsorption capacity was achieved with the following conditions: the concentration of extact liquid was 0. 125 g x mL(-1) (equivalence to raw material), then the resin was washed by water to remove impurity and alkaloids were desorbed by 50% ethanol.
CONCLUSION
This method is simple, feasible and suitable for industry production.
目的
通过选择合适的大孔吸附树脂，探索莲子心中生物碱的最佳分离方法。
方法
通过测定吸附率、洗脱率和甲基莲心碱的浓度来评估分离效率。
结果
LSA -5B大孔吸附树脂具有最佳的分离效率。在以下条件下可达到最佳吸附容量：提取液浓度为0.125 g·mL⁻¹（相当于原料），然后用水洗涤树脂以去除杂质，并用50%乙醇解吸生物碱。
结论
该方法简单、可行，适合工业化生产。
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