[四叶参水煎液总黄酮的分离纯化工艺]
[Separation and purification process of total flavones from four-leaf diet decoction].
作者信息
Wei Qiang, Yin Wei, Zhang Wen-Jing, Wu Yan
机构信息
Pharmacology College, Anhui Xinhua University, Hefei 230088, China.
出版信息
Zhong Yao Cai. 2011 Oct;34(10):1612-4.
OBJECTIVE
To investigate purification process of total flavonoids from four-leaf diet decoction with macroreticular resin.
METHODS
By using the static absorption tests and dynamic absorption tests with different types of macroreticular resins, the better parameters were found and the orthogonal design were used in purified craft.
RESULTS
AB-8 resin showed the best absorption property with the following technological conditions: the ratio of resin (g) - extract (mL) is 1.2: 1, the ratio of column diameter to height was 1: 12, then washed with water to colorless, and the volume of 80% ethanol was 7 BV.
CONCLUSION
The process is stable and the purity of total flavones is up to 85%.
目的
研究大孔树脂纯化四叶参水煎液中总黄酮的工艺。
方法
通过不同型号大孔树脂的静态吸附试验和动态吸附试验，筛选出较优参数，并采用正交设计法进行纯化工艺研究。
结果
AB-8树脂对总黄酮的吸附性能最佳，其工艺条件为：树脂（g）-提取物（mL）比例为1.2∶1，柱径与柱高比为1∶12，用水洗至无色，80%乙醇洗脱体积为7 BV。
结论
该工艺稳定，总黄酮纯度可达85%。
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