Ungureanu Carmen, Teleman S, Socolov Demetra, Anton Gabriela, Mihailovici Maria-Sultana
Disciplina de Morfopatologie, Facultatea de Medicină, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2010 Jul-Sep;114(3):823-8.
The aim of our study was to evaluate the expression of p16INK4A and Ki-67 in cervical intraepithelial neoplasia (CIN) and to correlate the high-risk human papillomavirus (HPV) infection with these two biomarkers.
Sixty patients were included in our study. All of the patient underwent colposcopy-guided biopsy to asses the grade of CIN. For the immunohistochemistry exam we used p161NK4A and Ki-67 antibody and their expression was semiquantitatively classified in 4 classes: 0, 1, 2 and 3. HPV genotyping was used to detect high-risk HPV.
7 of our cases were benign (11.66%); 18 cases presented CIN1 (30%), 14 cases CIN2 (23.33%), 16 cases CIN3 (26.66%) and 5 cases invasive squamous cell carcinoma (8.33%). We observed that the expression of p16INK4A and Ki-67 were positively associated with CIN grade, p161NK4A expressions increased significantly with high-risk HPV infection. We observed a positive correlation between the expression of the p16INK4A and Ki-67.
In our study, the expression of p16 and Ki-67 were positively related to the CIN grade. p16INK4A expressions of high-risk HPV specimens significantly increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV infection, p16INK4A can be a useful biomarker.
本研究旨在评估p16INK4A和Ki-67在宫颈上皮内瘤变（CIN）中的表达，并将高危型人乳头瘤病毒（HPV）感染与这两种生物标志物相关联。
本研究纳入60例患者。所有患者均接受阴道镜引导下活检以评估CIN分级。免疫组织化学检查采用p16INK4A和Ki-67抗体，其表达半定量分为4级：0、1、2和3级。采用HPV基因分型检测高危型HPV。
7例为良性（11.66%）；18例为CIN1（30%），14例为CIN2（23.33%），16例为CIN3（26.66%），5例为浸润性鳞状细胞癌（8.33%）。我们观察到p16INK4A和Ki-67的表达与CIN分级呈正相关，p16INK4A表达随高危型HPV感染显著增加。我们观察到p16INK4A与Ki-67的表达呈正相关。
在本研究中，p16和Ki-67的表达与CIN分级呈正相关。高危型HPV标本的p16INK4A表达显著高于Ki-67。因此，在CIN和高危型HPV感染的诊断中，p16INK4A可作为一种有用的生物标志物。
